Molecular bases of unexpressed RHD alleles in Chinese D− persons

  title={Molecular bases of unexpressed RHD alleles in Chinese D− persons},
  author={L Y Ye and D‐Q. Yue and de Jager Wo and Xiyu Ding and Shuangmei Guo and Qin Li and Zhong-hui Guo and Ziyan Zhu},
BACKGROUND: The aim of this study was to use a systematic survey to analyze RHD alleles in Chinese D− donors who do not express D antigen or who lack functional RhD protein. 

Characterization of novel RHD alleles: relationship between phenotype, genotype, and trimeric architecture

This data indicates that the presence of two new variant RHD alleles in the blood stream is a cause for concern, but the prognosis is good for both transfusion and foetal protection.

Weak D phenotypes caused by intronic mutations in the RHD gene: four novel weak D alleles identified in the Chinese population

Although more than 80 weak D types have been reported, many rare alleles probably remain unidentified. However, direct evidence that associates intronic mutations in the RHD gene with weak D types is

Weak D and DEL alleles detected by routine SNaPshot genotyping: identification of four novel RHD alleles

The purpose of the work was to develop a multiplex polymerase chain reaction SNaPshot assay for simultaneous detection of weak D and DEL alleles that are prevalent in Europeans, Africans, and Asians.

RHD genotype and zygosity analysis in the Chinese Southern Han D+, D− and D variant donors using the multiplex ligation‐dependent probe amplification assay

The multiplex ligation‐dependent probe amplification (MLPA) assay was validated for RHD genotyping in the Chinese.

Partial D phenotypes and genotypes in the Chinese population

This work has shown that the molecular basis underlying the partial D phenotype has been investigated in several races, but data from Chinese populations are rare and this work is likely to be a first of its kind.

WITHDRAWN: Rh genotypes among Malaysian blood donors.

  • R. H. MusaA. HassanY. AyobN. Yusoff
  • Medicine
    Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis
  • 2014

Molecular analysis of Malaysian Chinese D- donors: a single centre experience

A wide variation in the incidence of D- as well as the distribution of various D-phenotypes among the three major ethnic groups is found in the donor population.

Red blood cell genotyping in China

‘Asian type’ DEL allele (RHD*DEL1 defined by 1227G>A, Lys409Lys) is the most common variant and is classified as D‐, and it can only be identified by absorption/elution testing, which is not routinely conducted.

A comprehensive investigation of RHD polymorphisms in the Chinese Han population in Xi'an.

It seems to be difficult to observe any new RHD alleles in the Han Chinese population, as four new weak D alleles were noted, and two cases of RHD(1227G>A)/weak D type 15 heterozygosity were confirmed.

Prospective Evaluation of a Transfusion Policy of RhD-Positive Red Blood Cells into DEL Patients in China

Evidence is provided that different DEL genotypes code either for partial or complete D antigen expression, and it is suggested that the use of RhD+ RBCs incomplete D antigen DEL patients does not induce adverse reaction.



Molecular and family analyses revealed two novel RHD alleles in a survey of a Chinese RhD‐negative population

The purpose of the present work was to elucidate the molecular bases of two novel RHD alleles identified in a survey of a Chinese RhD‐negative population.

Molecular background of Rh D‐positive, D‐negative, Del and weak D phenotypes in Chinese

The genetic background of D‐negative and Del in the Chinese population is elucidated and it is found that D‐ negative and Del are more common in the female population than the male population.

Presence of RHD in serologically D–, C/E+ individuals: a European multicenter study

RHD blood group alleles with reduced or absent antigen expression are a clinically significant and heterogeneous group and should be considered a clinical indication for further studies.

Systemic analysis and zygosity determination of the RHD gene in a D‐negative Chinese Han population reveals a novel D‐negative RHD gene

The aim of this study was to systemically analyse the genetic background of D negativity in a Chinese Han population and found that D negativity is higher in the Han population than in other ethnic groups.

Molecular characterization of D– Korean persons: development of a diagnostic strategy

D– frequencies show wide racial differences in Korean persons, and it was hypothesized that D– frequencies may be affected by genomic variations of Rhesus boxes, which are instruments of unequal crossing‐over.

Molecular basis for the RhD negative phenotype in Chinese.

The RhD negative variants found in this study are combination of two of the three alleles, described above, which are not reliable methods for studies in the Chinese population.

Analysis of RHD genes in Taiwanese RhD‐negative donors by the multiplex PCR method

Six defined RhD variants were found in this study: four ROHar, one DVa, and two DIVb, however, no true RhD‐negative or RhDel donor with the CcdEe phenotype was found inThis analysis.

RHD positive haplotypes in D negative Europeans

The results of this study allowed to devise an improved RHD genotyping strategy, the false-positive rate of which was lower than 1:10,000 and the number of characterized RHD positive antigen D negative and Del alleles was more than doubled and their population frequencies in Europe were defined.

DNA‐Based Rhesus Typing: Simultaneous Determination of RHC and RHD Status Using the Polymerase Chain Reaction

It is shown that the PCR‐based method of performing RHD and C/c typing in a single reaction has potential in the antenatal determination of RH type, as it was possible to type fetal trophoblasts recovered from the endocervical canal at 9 weeks pregnancy.

The presence of an RHD pseudogene containing a 37 base pair duplication and a nonsense mutation in africans with the Rh D-negative blood group phenotype.

Existing PCR-based methods for predicting D phenotype from DNA are not suitable for testing Africans or any population containing a substantial proportion of people with African ethnicity, so a new test is developed that detects the 37 bp insert in exon 4 of RHDpsi.