Molecular-based detection of non-culturable and emerging campylobacteria in patients presenting with gastroenteritis.

  title={Molecular-based detection of non-culturable and emerging campylobacteria in patients presenting with gastroenteritis.},
  author={Susan Bullman and James O'Leary and Daniel G Corcoran and Roy D. Sleator and Brigid Lucey},
  journal={Epidemiology and infection},
  volume={140 4},
From January 2009 to May 2010, 436 faecal samples from patients with diarrhoeal illness in Southern Ireland were identified as Campylobacter genus-positive by an automated multiplex PCR; however, 204 (46·8%) of these samples were culture-negative for campylobacters. A combination of Campylobacter-specific uniplex PCR and 16S rRNA sequencing confirmed the presence of Campylobacter DNA in 191 (93·6%) of the culture-negative samples. Species-specific PCR identified C. jejuni (50·7%) C. ureolyticus… CONTINUE READING
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