Molecular barcodes for soil nematode identification

  title={Molecular barcodes for soil nematode identification},
  author={R. Floyd and Eyualem Abebe and A. Papert and M. Blaxter},
  journal={Molecular Ecology},
  • R. Floyd, Eyualem Abebe, +1 author M. Blaxter
  • Published 2002
  • Biology, Medicine
  • Molecular Ecology
  • Using a molecular barcode, derived from single‐specimen polymerase chain reaction (PCR) and sequencing of the 5′ segment of the small subunit ribosomal RNA (SSU) gene, we have developed a molecular operational taxonomic unit (MOTU) scheme for soil nematodes. Individual specimens were considered to belong to the same MOTU when the sequenced segment of 450 bases was > 99.5% identical. A Scottish upland Agrostis‐Festuca grassland soil was sampled, using both culture‐based and random selection… CONTINUE READING
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