• DOI:10.1046/j.1365-294X.2002.01485.x
Corpus ID: 12955921

Molecular barcodes for soil nematode identification

@article{Floyd2002MolecularBF,
  title={Molecular barcodes for soil nematode identification},
  author={R. Floyd and Eyualem Abebe and A. Papert and M. Blaxter},
  journal={Molecular Ecology},
  year={2002},
  volume={11}
}

R. Floyd, Eyualem Abebe, +1 author M. Blaxter

Published 2002

Biology, Medicine

Molecular Ecology

Using a molecular barcode, derived from single‐specimen polymerase chain reaction (PCR) and sequencing of the 5′ segment of the small subunit ribosomal RNA (SSU) gene, we have developed a molecular operational taxonomic unit (MOTU) scheme for soil nematodes. Individual specimens were considered to belong to the same MOTU when the sequenced segment of 450 bases was > 99.5% identical. A Scottish upland Agrostis‐Festuca grassland soil was sampled, using both culture‐based and random selection… CONTINUE READING

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