Molecular aspects of gene transfer and foreign DNA acquisition in prokaryotes with regard to safety issues

@article{Brigulla2010MolecularAO,
  title={Molecular aspects of gene transfer and foreign DNA acquisition in prokaryotes with regard to safety issues},
  author={Matthias Brigulla and Wilfried Wackernagel},
  journal={Applied Microbiology and Biotechnology},
  year={2010},
  volume={86},
  pages={1027-1041}
}
Horizontal gene transfer (HGT) is part of prokaryotic life style and a major factor in evolution. In principle, any combinations of genetic information can be explored via HGT for effects on prokaryotic fitness. HGT mechanisms including transformation, conjugation, transduction, and variations of these plus the role of mobile genetic elements are summarized with emphasis on their potential to translocate foreign DNA. Complementarily, we discuss how foreign DNA can be integrated in recipient… 

Exploration of horizontal gene transfer between transplastomic tobacco and plant-associated bacteria.

The results provide no indication that the theoretical gene transfer-enhancing properties of transplastomic plants cause horizontal gene transfer at rates above those found in other studies with nuclear transgenes.

Mycoplasma Chromosomal Transfer: A Distributive, Conjugative Process Creating an Infinite Variety of Mosaic Genomes

This study unraveled the tremendous plasticity of M. agalactiae genome and point toward MCT as a major player in diversification and adaptation to changing environments, offering a significant advantage to this minimal pathogen.

Horizontal gene transfer from genetically modified plants - Regulatory considerations

This review provides updated evidence on the likelihood, factors and the barriers for the introduced or modified DNA in GM plants to be horizontally transferred into a variety of recipients and presents the legislation and frameworks the Australian Gene Technology Regulator adheres to.

The Extent and Regulation of Lateral Gene Transfer in Natural Microbial Ecosystems

The discovery and application of antibiotics beginning from the early 1940s has revolutionized the branch of clinical microbiology aimed at control and treatment of infectious diseases and allowed humans to adapt these mechanisms to serve their own needs.

Recombination proteins differently control the acquisition of homeologous DNA during Bacillus subtilis natural chromosomal transformation.

It is proposed that DprA, RecX, RadA/Sms, RecJ and RecD2 accessory proteins are important for the generation of genetic diversity together with RecA, they facilitate gene acquisition from bacteria of related species.

Plasmid-Mediated Transfer of Antibiotic Resistance Genes in Soil

The current scenario of plasmid-mediated migration and transmission of ARGs in natural environments and under different antibiotic selection pressures is reviewed, the current methods of plasmsid extraction and analysis are summarized, and the mechanism ofplasmid splice transfer using the F factor as an example is introduced.

Bacillus subtilis MutS Modulates RecA-Mediated DNA Strand Exchange Between Divergent DNA Sequences

The data supports the idea that MutS modulates bidirectional RecA-mediated integration of divergent sequences and this is important for speciation, and suggests that homology-facilitated illegitimate recombination might aid in the restoration of inactivated genes.

Horizontal transfer of antibiotic resistance genes into microflora and blood cells in rats fed on GM-diet

The occurrence of DNA transfer of nptII and aadA genes from GM-diet to blood and bacterial cells has been unambiguously demonstrated and may draw attentions to the importance of exploring the possible effects of transfer of ARM genes horizontally from GM products to consumers.
...

References

SHOWING 1-10 OF 177 REFERENCES

Integration of foreign DNA during natural transformation of Acinetobacter sp. by homology-facilitated illegitimate recombination

It is found that the integration of foreign DNA into the genome of the Gram-negative Acinetobacter sp.

Risks from GMOs due to horizontal gene transfer.

  • P. Keese
  • Biology
    Environmental biosafety research
  • 2008
The genome of almost every organism reveals the footprint of many ancient HGT events, and in most cases the occurrence of HGT from GM crops to other organisms is expected to be lower than background rates, therefore, H GT from GM plants poses negligible risks to human health or the environment.

Microbial horizontal gene transfer and the DNA release from transgenic crop plants

The results suggest that, although such transfers could be possible in principle, each of the many steps involved from the release of intact DNA from a plant cell to integration into a prokaryotic genome has such a low probability that a successful transfer event be extremely rare.

Shaping bacterial genomes with integrative and conjugative elements.

Shuffling genes around in hot environments: the unique DNA transporter of Thermus thermophilus.

It is hypothesize that the extraordinary broad substrate specificity and the high efficiency of the T. thermophilus DNA uptake system is of major importance for thermoadaptation and interdomain DNA transfer in hot environments.

Bacterial gene transfer by natural genetic transformation in the environment.

The current understanding of the biology of transformation is summarized to provide the platform on which aspects of bacterial transformation in water, soil, and sediments and the habitat of pathogens are discussed.

Genomic islands: tools of bacterial horizontal gene transfer and evolution

Genomic islands play a crucial role in the evolution of a broad spectrum of bacteria as they are involved in the dissemination of variable genes, including antibiotic resistance and virulence genes leading to generation of hospital ‘superbugs’, as well as catabolic genes lead to formation of new metabolic pathways.

Horizontal gene transfer in metal and radionuclide contaminated soils.

The horizontal transfer of genes encoded on mobile genetic elements (MGEs) such as plasmids and phage and their associated hitchhiking elements (transposons, integrons, integrative and conjugative

Double illegitimate recombination events integrate DNA segments through two different mechanisms during natural transformation of Acinetobacter baylyi

The HFDIR phenomenon indicates that a temporal recA‐dependent association of donor DNA at a homology in recipient DNA may facilitate two IR events on the 5′ and 3′ heterologous parts of the transforming DNA molecule.

Transfer of plastid DNA from tobacco to the soil bacterium Acinetobacter sp. by natural transformation

The results show that integration of plant DNA into a bacterial genome by natural transformation is possible and is probably stimulated by hot‐spots of illegitimate recombination.
...