Molecular architecture of the kinetochore–microtubule interface

  title={Molecular architecture of the kinetochore–microtubule interface},
  author={Iain M. Cheeseman and Arshad Desai},
  journal={Nature Reviews Molecular Cell Biology},
Segregation of the replicated genome during cell division in eukaryotes requires the kinetochore to link centromeric DNA to spindle microtubules. The kinetochore is composed of a number of conserved protein complexes that direct its specification and assembly, bind to spindle microtubules and regulate chromosome segregation. Recent studies have identified more than 80 kinetochore components, and are revealing how these proteins are organized into the higher order kinetochore structure, as well… 
The kinetochore.
  • I. Cheeseman
  • Biology
    Cold Spring Harbor perspectives in biology
  • 2014
This review will discuss the key kinetochore activities required for mitotic chromosome segregation, including the recognition of a specific site on each chromosome, kinETochore assembly and the formation of kinetic-microtubule connections, and the generation of force to drive chromosome segregation.
Establishment of the vertebrate kinetochores
Recent progress with regard to the molecular architecture of the kinetochore is reviewed and the future directions for centromere biology are discussed.
Kinetochore assembly: if you build it, they will come.
Kinetochore-microtubule interactions: the means to the end.
The kinetochore and spindle checkpoint in vertebrate cells.
  • T. Fukagawa
  • Biology
    Frontiers in bioscience : a journal and virtual library
  • 2008
Recent advances in the understanding of the kinetochore and spindle checkpoint in vertebrate cells are reviewed and their implications using chicken DT40 system are discussed.
Subunit organization in the Dam1 kinetochore complex and its ring around microtubules
Mapping of subunits within the Dam1 complex and their organization within the ring has made it possible to generate a model of Dam1 ring assembly.


Structure, function, and regulation of budding yeast kinetochores.
This review focuses on the simplest eukaryotic centromeres and kinetochores, those found in the budding yeast Saccharomyces cerevisiae.
Structures and functions of yeast kinetochore complexes.
The current understanding of kinetochore function in budding yeast is reviewed and comparisons to recent findings in other organisms are drawn.
The spindle-assembly checkpoint in space and time
Recent molecular analyses have begun to shed light on the complex interaction of the checkpoint proteins with kinetochores — structures that mediate the binding of spindle microtubules to chromosomes in mitosis.
Ran at kinetochores.
  • M. Dasso
  • Biology
    Biochemical Society transactions
  • 2006
The Ran GTPase controls many cellular functions, including nucleocytoplasmic trafficking, spindle assembly, nuclear assembly and cell-cycle progression, and has two important but less understood roles at mitotic kinetochores.
Genes Required for Mitotic Spindle Assembly in Drosophila S2 Cells
Using a full-genome RNA interference screen of Drosophila S2 cells, about 200 genes that contribute to spindle assembly are identified, more than half of which were unexpected.
Protein arms in the kinetochore-microtubule interface of the yeast DASH complex.
The microtubule-binding properties of limited proteolysis products and subcomplexes of DASH are characterized, thus identifying candidate polypeptide extensions involved in establishing the DASH-microtubule interface and measuring the molecular mass of Dash rings on microtubules with scanning transmission electron microscopy.
Molecular architecture of a kinetochore–microtubule attachment site
It is found that relative to the two Cse4p molecules in the centromeric histone, the copy number ranges from one or two for inner kinetochore proteins such as Mif2p, to 16 for the DAM–DASH complex at the kinet Cochore–microtubule interface.
Ran-GTP regulates Kinetochore Attachment in Somatic Cells
The Ran GTPase controls multiple mitotic processes in Xenopus egg extracts, including mitotic checkpoints, spindle assembly and post-mitotic nuclear envelope reassembly, and a novel mitotic role of Ran-GTP is uncovered, involving the Crm1 nuclear export receptor.
"Holo"er than thou: Chromosome segregation and kinetochore function in C. elegans
The nature of chromosome segregation in C. elegans is discussed and the consequences of the specialized holocentric architecture on chromosome segregation are considered.