Molecular architecture of the kinetochore–microtubule interface

@article{Cheeseman2008MolecularAO,
  title={Molecular architecture of the kinetochore–microtubule interface},
  author={Iain M. Cheeseman and Arshad Desai},
  journal={Nature Reviews Molecular Cell Biology},
  year={2008},
  volume={9},
  pages={33-46}
}
Segregation of the replicated genome during cell division in eukaryotes requires the kinetochore to link centromeric DNA to spindle microtubules. The kinetochore is composed of a number of conserved protein complexes that direct its specification and assembly, bind to spindle microtubules and regulate chromosome segregation. Recent studies have identified more than 80 kinetochore components, and are revealing how these proteins are organized into the higher order kinetochore structure, as well… 
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836 Citations

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This review will discuss the key kinetochore activities required for mitotic chromosome segregation, including the recognition of a specific site on each chromosome, kinETochore assembly and the formation of kinetic-microtubule connections, and the generation of force to drive chromosome segregation.
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Recent advances in the understanding of the kinetochore and spindle checkpoint in vertebrate cells are reviewed and their implications using chicken DT40 system are discussed.
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Mapping of subunits within the Dam1 complex and their organization within the ring has made it possible to generate a model of Dam1 ring assembly.
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