Molecular approaches for viable bacterial population and transcriptional analyses in a rodent model of dental caries.

@article{Klein2012MolecularAF,
  title={Molecular approaches for viable bacterial population and transcriptional analyses in a rodent model of dental caries.},
  author={Marlise In{\^e}z Klein and Kathleen Scott-Anne and Stacy Gregoire and Pedro Luiz Rosalen and Hyun Koo},
  journal={Molecular oral microbiology},
  year={2012},
  volume={27 5},
  pages={350-61}
}
Culturing methods are the primary approach for microbiological analysis of plaque biofilms in rodent models of dental caries. In this study, we developed strategies for the isolation of DNA and RNA from plaque biofilms formed in vivo to analyse the viable bacterial population and gene expression. Plaque biofilm samples from rats were treated with propidium monoazide to isolate DNA from viable cells, and the purified DNA was used to quantify total bacteria and the Streptococcus mutans population… CONTINUE READING
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