Molecular and physiological evaluation of transgenic tobacco plants expressing a maize phosphoenolpyruvate carboxylase gene under the control of the cauliflower mosaic virus 35S promoter

@article{Kogami2005MolecularAP,
  title={Molecular and physiological evaluation of transgenic tobacco plants expressing a maize phosphoenolpyruvate carboxylase gene under the control of the cauliflower mosaic virus 35S promoter},
  author={Hiroyuki Kogami and Mariko Shono and Takayoshi Koike and Syuuichi Yanagisawa and K. Izui and Naoki Sentoku and Shigeyuki Tanifuji and Hirofumi Uchimiya and Seiichi Toki},
  journal={Transgenic Research},
  year={2005},
  volume={3},
  pages={287-296}
}
The expression of maize (Zea mays) phosphoenolpyruvate carboxylase (PEPC) gene constructs in transgenic tobacco plants (Nicotiana tabacum) was studied. Where transcription was under the control of a CaMV 35S promoter, maize PEPC transcripts of the correct size were detected. Western blot analysis indicated that the transgenic plants contained about twice as much PEPC as non-transformed plants. Furthermore, the enzymatic activity of PEPC in the leaves of these transgenic plants was up to twice… CONTINUE READING