Molecular and immunological characterization of β'-component (Onc k 5), a major IgE-binding protein in chum salmon roe.

@article{Shimizu2014MolecularAI,
  title={Molecular and immunological characterization of β'-component (Onc k 5), a major IgE-binding protein in chum salmon roe.},
  author={Yutaka Shimizu and Hideki Kishimura and Gaku Kanno and Atsushi Nakamura and Reiko Adachi and Hiroshi Akiyama and Kazuhiko Watanabe and Akihiko Hara and Motohiro Ebisawa and Hiroki Saeki},
  journal={International immunology},
  year={2014},
  volume={26 3},
  pages={139-47}
}
Salmon roe has a high allergic potency and often causes anaphylaxis in Japan. The major allergic protein of salmon roe is β'-component, which is a 35kDa vitellogenin fragment consisting of two subunits. To elucidate structural information and immunological characteristics, β'-component and the subunit components were purified from chum salmon (Onchorhincus keta) roe and vitellogenin-encoding mRNA was used to prepare β'-component subunit-encoding cDNA. This was PCR-amplified, cloned and… CONTINUE READING
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