Molecular and histomorphological evaluation of female rats’ urethral tissues after an innovative trauma model of prolonged vaginal distention: immediate, short-term and long-term effects

  title={Molecular and histomorphological evaluation of female rats’ urethral tissues after an innovative trauma model of prolonged vaginal distention: immediate, short-term and long-term effects},
  author={M. Bortolini and S. M. Feitosa and A. P. M. Bilhar and G. Salerno and E. Zanoteli and M. Sim{\~o}es and R. Castro},
  journal={International Urogynecology Journal},
Introduction and hypothesisAn animal model of vaginal distention (VD) was developed to reproduce the acute urethral injury and deficiency underlying stress urinary incontinence (SUI). Data on the chronic effects of urethral trauma and the recovery process are still scarce. We investigated acute, short- and long-term histomorphological and molecular changes in the urethra of rats post 12-h intermittent VD.MethodsWe evaluated the urethra of four groups of female rats (n = 72): control without… Expand
Long-term effects of muscle-derived stem cell therapy on the regeneration of the urethra of female rats
It is suggested that MDSC therapy acted mainly to produce urethral sphincter regeneration marked by increased immunohistochemical expression of the proteins desmin, smooth muscle Myh11, and skeletal Muscle Myh2. Expand
The molecular effects of electrical stimulation on the muscle components of the urethra of female rats after trauma by vaginal distention
To evaluate the expression of genes and proteins related to the urethral muscles of female rats after trauma by vaginal distention (VD) and after electrical stimulation therapy (EST).
Electrotherapy for urethral modulation: Are extracellular matrix molecules and growth factors potential targets?
To evaluate the expression of genes and proteins involved in the urethral components: vessels, nerves, and extracellular matrix, in female rats after trauma by vaginal distension (VD) and afterExpand
Neurourology and Urodynamics
Department of Gynecology, Sector of Urogynecology and Vaginal Surgery, Universidade Federal de São Paulo, São Paulo, Brazil Histology and Structural Biology Division, Department of Morphology andExpand


Analysis of primary urethral wound healing in the rat.
Urethral healing occurs in phases similar to those observed in dermal healing, however, with extension of each phase, and the healing process is not limited to the site of injury but involves the vast majority of periurethral tissue and corpus spongiosum. Expand
Comparison of three types of stress urinary incontinence rat models: electrocauterization, pudendal denervation, and vaginal distension.
The vaginal distension model should mainly be used as the myogenicdamage stress urinary incontinence animal model; the pudendal denervation model mainly as the neurogenic damage stress urinaryincontinenceAnimal model; and the electrocauterization model as the vasculogenic, neurogenic, and myogenic damage animal model. Expand
Impact of parturition on chemokine homing factor expression in the vaginal distention model of stress urinary incontinence.
Vaginal distention results in up-regulation of the chemokines and receptors expressed during tissue injury, which may facilitate the spontaneous functional recovery previously noted. Expand
Ex vivo biomechanical properties of the female urethra in a rat model of birth trauma.
Biomechanical analyses suggest that VD alters Urethral basal tone, proximal urethral compliance, and distal stiffness, and Lack of basal smooth muscle tone, in combination with these changes in the proximal anddistal urethra, may contribute to SUI induced by VD. Expand
Vulnerability of continence structures to injury by simulated childbirth.
The EUS underwent the greatest insult, demonstrating its vulnerability to childbirth injury and morphological changes in the urethra and vagina with increasing duration of vaginal distension. Expand
Over expression of stem cell homing cytokines in urogenital organs following vaginal distention.
Rat models of simulated childbirth demonstrated hypoxia of the urogenital organs as well as the development of stress urinary incontinence following vaginal distention, and monocyte chemotactic protein-3 is significantly over expressed in rat urethral and vaginal tissues immediately following vaginal Distention with above normal but decreasing expression 24 hours later. Expand
Cytokine expression after vaginal distention of different durations in virgin Sprague-Dawley rats.
Data support a relationship between vaginal distention duration and the subsequent expression of monocyte chemotactic protein-3 and 1 of its associated receptors, CCR1, in the urethra immediately following vaginaldistention. Expand
Increased duration of simulated childbirth injuries results in increased time to recovery.
Pressure-induced hypoxia is suggested as a possible mechanism of injury in vaginal delivery after increased duration of vaginal distension on voiding cystometry, leak point pressure testing, and histology. Expand
The impact of pregnancy and childbirth in the urethra of female rats
Pregnancy and cesarean did not induce alterations in collagen, muscular, and elastic fibers, but the vaginal delivery and simulation of childbirth trauma determined the decrease in muscular fibers and the increase in collagen and elastic Fiber correlation. Expand
Dual simulated childbirth injury delays anatomic recovery.
PNC+VD provides a more severe injury than PNC or VD alone, and all injury groups had fewer normal nerve fascicles, but without significant differences compared with C rats. Expand