Molecular and genetic characterization of an Alcaligenes eutrophus insertion element.

@article{Kung1992MolecularAG,
  title={Molecular and genetic characterization of an Alcaligenes eutrophus insertion element.},
  author={Susan Smythe Kung and John W. Chen and Wei Yuan Chow},
  journal={Journal of bacteriology},
  year={1992},
  volume={174 24},
  pages={
          8023-9
        }
}
An insertion element, ISAE1, was discovered during the molecular analysis of mutants defective in the autotrophic growth (Aut-) of Alcaligenes eutrophus H1-4, a mitomycin C-generated derivative of strain H1. ISAE1 is 1,313 bp long, has 12-bp nearly perfect inverted terminal repeats, and contains an open reading frame that has a coding capacity of 408 amino acids. Direct repeats of 8 bp were generated by insertion of ISAE1 into chromosomes or plasmids. Most insertion were found in the AT-rich… CONTINUE READING

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