Molecular and functional evidence for multiple Ca2+-binding domains in the type 1 inositol 1,4,5-trisphosphate receptor.

@article{Sienaert1997MolecularAF,
  title={Molecular and functional evidence for multiple Ca2+-binding domains in the type 1 inositol 1,4,5-trisphosphate receptor.},
  author={Ilse Sienaert and Ludwig Missiaen and Humbert de Smedt and Jan B Parys and Henk Sipma and Rik Casteels},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 41},
  pages={25899-906}
}
Structural and functional analyses were used to investigate the regulation of the inositol 1,4,5-trisphosphate (InsP3) receptor (InsP3R) by Ca2+. To define the structural determinants for Ca2+ binding, cDNAs encoding GST fusion proteins that covered the complete linear cytosolic sequence of the InsP3R-1 were expressed in bacteria. The fusion proteins were screened for Ca2+ and ruthenium red binding through the use of 45Ca2+ and ruthenium red overlay procedures. Six new cytosolic Ca2+-binding… CONTINUE READING

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