Molecular and functional characterization of a murine calcium-activated chloride channel expressed in smooth muscle.

@article{Elble2002MolecularAF,
  title={Molecular and functional characterization of a murine calcium-activated chloride channel expressed in smooth muscle.},
  author={Randolph C Elble and Guangju Ji and Keith Nehrke and John DeBiasio and Paul Kingsley and Michael I. Kotlikoff and Bendicht U. Pauli},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 21},
  pages={18586-91}
}
To identify the gene products responsible for the calcium-activated chloride current in smooth muscle, reverse transcription-PCR with degenerate primers was performed on mouse intestine and other organs. A new member of the CLCA gene family was identified, mCLCA4, that is expressed preferentially in organs containing a high percentage of smooth muscle cells, including intestine, stomach, uterus, bladder, and aorta. Reverse transcription-PCR using template RNA prepared from mouse bladder and… CONTINUE READING

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