Molecular and catalytic properties of a novel cytochrome c nitrite reductase from nitrate-reducing haloalkaliphilic sulfur-oxidizing bacterium Thioalkalivibrio nitratireducens.

@article{Tikhonova2006MolecularAC,
  title={Molecular and catalytic properties of a novel cytochrome c nitrite reductase from nitrate-reducing haloalkaliphilic sulfur-oxidizing bacterium Thioalkalivibrio nitratireducens.},
  author={Tamara V Tikhonova and Alvira Slutsky and Alexey N. Antipov and Konstantin M. Boyko and Konstantin M Polyakov and Dimitry Yu Sorokin and R. A. Zvyagilskaya and Vladimir O. Popov},
  journal={Biochimica et biophysica acta},
  year={2006},
  volume={1764 4},
  pages={715-23}
}
A highly active cytochrome c nitrite reductase from the haloalkaliphilic sulfur-oxidizing non-ammonifying bacterium Tv. nitratireducens strain ALEN 2 (TvNiR) was isolated and purified to apparent electrophoretic homogeneity. The enzyme catalyzes reductive conversion of nitrite and hydroxylamine to ammonia without release of any intermediates, as well as reduction of sulfite to sulfide. TvNiR also possesses peroxidase activity. In solution TvNiR exists as a stable hexamer with molecular mass of… CONTINUE READING