Toxoplasma gondii serine hydrolases regulate parasite lipid mobilization during growth and replication within the host
Bioinformatic and biochemical characterization of T. gondii serine hydrolases reveals substrate preference between enzymes with similar catalytic fold and shows that treatment of parasites results in phenotypic defects reminiscent of parasites exposed to excess cholesterol or oleic acid.
Functional characterization of single-domain cystatin-like cysteine proteinase inhibitors expressed by the trematode Fasciola hepatica
The aim of this work is to characterize the cystatin repertoire of F. hepatica, and to suggest that it is secreted by non-classical secretory pathway and that it may interact with host lysosomal cysteine proteinases.
Evaluation of the immunodiagnostic potential of a recombinant surface protein domain from Acanthamoeba castellanii
- Biology, MedicineParasitology
The results showed the AcCRT29–130 potential for A. castellanii infection immunodiagnosis in animals, with further studies being required for assessment of its use for human infections.
The animal trypanosomiases and their chemotherapy: a review
This review provides a comprehensive overview of the current options available for the treatment and prophylaxis of the animal trypanosomiases, with a special focus on the problem of resistance.
FhCaBP2: a Fasciola hepatica calcium-binding protein with EF-hand and dynein light chain domains
Overall, FhCaBP2 has distinct biochemical properties compared with other members of this protein family from Fasciola hepatica, a fact which supports the hypothesis that these proteins have different physiological roles.
The gatekeeper residue and beyond: homologous calcium-dependent protein kinases as drug development targets for veterinarian Apicomplexa parasites
Divergent BKI sensitivity of CDPK homologues could be explained on the basis of differences in the size and orientation of the hydrophobic pocket and specific variation at other amino-acid positions within the ATP-binding cavity.
Next-generation sequencing reveals cryptic mtDNA diversity of Plasmodium relictum in the Hawaiian Islands
The discovery of multiple mitochondrial lineages of P. relictum in Hawaii provides a measure of genetic diversity within a geographically isolated population of this parasite and suggests the origins and evolution of parasite diversity may be more complicated than previously recognized.
Anthelmintic metabolism in parasitic helminths: proteomic insights
- Medicine, BiologyParasitology
Optimisations of membrane, post-translational modification and interaction proteomic technologies in helminths are needed to especially study Phase I CYPs and Phase III ABC transporter pumps for anthelmintics and their metabolites.
Considering RNAi experimental design in parasitic helminths
Multiple approaches to RNAi experimental validation are identified and highlighted those which are deemed to be critical for gene function studies in helminth parasites.