Molecular and biochemical analysis of a Madagascar periwinkle root-specific minovincinine-19-hydroxy-O-acetyltransferase.

@article{Laflamme2001MolecularAB,
  title={Molecular and biochemical analysis of a Madagascar periwinkle root-specific minovincinine-19-hydroxy-O-acetyltransferase.},
  author={Philippe Laflamme and Benoit St-Pierre and V DeLuca},
  journal={Plant physiology},
  year={2001},
  volume={125 1},
  pages={189-98}
}
The terminal steps in the biosynthesis of the monoterpenoid indole alkaloids vindoline and minovincinine are catalyzed by separate acetyl coenzyme A-dependent O-acetyltransferases in Madagascar periwinkle (Catharanthus roseus G. Don). Two genes were isolated that had 63% nucleic acid identity and whose deduced amino acid sequences were 78% identical. Active enzymes that were expressed as recombinant His-tagged proteins in Escherichia coli were named minovincinine-19-O-acetyltransferase (MAT… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 23 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 20 references

Multi - cellular compartmentation of Catharanthus roseus alkaloid biosynthesis predicts intercellular translocation of a pathway intermediate

  • B St-Pierre, FA Vázquez-Flota, V DeLuca
  • Plant Cell
  • 1999
Highly Influential
5 Excerpts

Stable vindoline production in transformed cell cultures of Catharanthus roseus

  • BR O’Keefe, GB Mahady, JJ Gills, Beecher CWW
  • J Nat Prod
  • 1997
Highly Influential
4 Excerpts

Purification and characterization of acetylcoenzyme A : deacetylvindoline 4O - acetyltransferase from Catharanthus roseus

  • R Power, Kurz WGW, V DeLuca
  • Arch Biochem Biophys
  • 1990
Highly Influential
2 Excerpts

Similar Papers

Loading similar papers…