Molecular and Pharmacological Characterization of Serotonin 5-HT2α and 5-HT7 Receptors in the Salivary Glands of the Blowfly Calliphora vicina

@article{Rser2012MolecularAP,
  title={Molecular and Pharmacological Characterization of Serotonin 5-HT2$\alpha$ and 5-HT7 Receptors in the Salivary Glands of the Blowfly Calliphora vicina},
  author={Claudia R{\"o}ser and Nadine Jordan and Sabine Balfanz and Arnd Baumann and Bernd Walz and Otto Baumann and Wolfgang Blenau},
  journal={PLoS ONE},
  year={2012},
  volume={7}
}
Secretion in blowfly (Calliphora vicina) salivary glands is stimulated by the biogenic amine serotonin (5-hydroxytryptamine, 5-HT), which activates both inositol 1,4,5-trisphosphate (InsP3)/Ca2+ and cyclic adenosine 3′,5′-monophosphate (cAMP) signalling pathways in the secretory cells. In order to characterize the signal-inducing 5-HT receptors, we cloned two cDNAs (Cv5-ht2α, Cv5-ht7) that share high similarity with mammalian 5-HT2 and 5-HT7 receptor genes, respectively. RT-PCR demonstrated… Expand
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References

SHOWING 1-10 OF 66 REFERENCES
Functional characterisation of a 5-HT2 receptor cDNA cloned from Lymnaea stagnalis.
TLDR
A G-protein-coupled receptor resembling members of the 5-HT2 receptor subfamily was cloned from the mollusc Lymnaea stagnalis to construct a cDNA encoding an amino-terminally truncated receptor that appeared to be much better expressed in HEK293 cells. Expand
Pharmacological characterizations of recombinant human 5-HT1D1Dα and 5-HT1Dβ receptor subtypes coupled to adenylate cyclase inhibition in clonal cell lines: apparent differences in drug intrinsic efficacies between human 5-HT1D subtypes
TLDR
Data are consistent with the hypothesis that the two heterologous expression systems contain a differential amount of receptor reserve, as well as system-independent differences in the intrinsic efficacy of the tested compounds at the two human 5-HT1D receptors. Expand
Am5‐HT7: molecular and pharmacological characterization of the first serotonin receptor of the honeybee (Apis mellifera)
TLDR
This study marks the first comprehensive characterization of a serotonin receptor in the honeybee and should facilitate further analysis of the role(s) of the receptor in mediating the various central and peripheral effects of 5‐HT. Expand
Distribution of serotonin (5-HT) and its receptors in the insect brain with focus on the mushroom bodies: lessons from Drosophila melanogaster and Apis mellifera.
TLDR
In vivo expression pattern of a recently characterized 5-HT(1) receptor of the honeybee Apis mellifera (Am5- HT(1A)) in the mushroom bodies is reported here and current knowledge on the distribution of serotonin and serotonin receptor subtypes in the brain and specifically in the Mushroom bodies of the fruit fly Drosophila melanogaster and the honeybees is summarized. Expand
Characterization of the 5-HT1A receptor of the honeybee (Apis mellifera) and involvement of serotonin in phototactic behavior
TLDR
The data presented here mark the first comprehensive study—from gene to behavior—of a 5-HT1A receptor in the honeybee, paving the way for the eventual elucidation of additional roles of this receptor subtype in the physiology and behavior of this social insect. Expand
Inhibition of Serotonin 5-Hydroxytryptamine2C Receptor Function through Heterodimerization
TLDR
Inactive 5-HT2C receptors inhibit wild-type 5- HT2C receptor function by forming nonfunctional heterodimers expressed on the plasma membrane, consistent with a model in which one GPCR dimer binds two molecules of ligand and one G-protein and indicate that dimerization is essential for 5-ht receptor function. Expand
SEPARATE 5‐HYDROXYTRYPTAMINE RECEPTORS ON THE SALIVARY GLAND OF THE BLOWFLY ARE LINKED TO THE GENERATION OF EITHER CYCLIC ADENOSINE 3‘,5’‐MONOPHOSPHATE OR CALCIUM SIGNALS
TLDR
It is concluded that 5‐HT acts on two separate receptors, a 5‐ HT1 receptor acting through calcium and a 5-HT2 receptor which mediates its effects through cyclic AMP. Expand
The 5-Hydroxytryptamine(1A) Receptor Is Stably Palmitoylated, and Acylation Is Critical for Communication of Receptor with Gi Protein*
TLDR
It is verified that the mouse 5-hydroxytryptamine( 1A) (5-HT1A) receptor is modified by palmitic acid, which is covalently attached to the protein through a thioester-type bond, suggesting that palmitoylation occurs early after synthesis of the 5- HT1A receptor. Expand
SER‐7b, a constitutively active Gαs coupled 5‐HT7‐like receptor expressed in the Caenorhabditis elegans M4 pharyngeal motorneuron
TLDR
This is the first report of a Gαs coupled biogenic amine receptor in nematodes and the localization of SER‐7b in the M4 pharyngeal motorneuron suggests that SER‐ 7b may play a role in the regulation of pharynGEal pumping. Expand
Calcineurin activity augments cAMP/PKA-dependent activation of V-ATPase in blowfly salivary glands.
TLDR
It is proposed that calcineurin activity mediates cross talk between the inositol 1,4,5-trisphosphate/Ca(2+) and the cAMP/PKA signaling pathways, thereby augmenting the 5-HT-induced rise in cAMP and thus the camp/ PKA-mediated activation of V-ATPase. Expand
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