Molecular analysis of the period locus in Drosophila melanogaster and identification of a transcript involved in biological rhythms

  title={Molecular analysis of the period locus in Drosophila melanogaster and identification of a transcript involved in biological rhythms},
  author={Pranhitha Reddy and William A. Zehring and David A. Wheeler and V. Pirrotta and Christopher Hadfield and Jeffrey C. Hall and Michael Rosbash},

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An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates
The results of a search for sequences homologous to the per locus DNA in the genomic DNA of several species of vertebrates show an unusual, tandemly repeated sequence forming a portion of the 4.5-kb per transcript is homologously to DNA in chicken, mouse and man.
Product of per locus of Drosophila shares homology with proteoglycans
A fragment of DNA of ∼7 kilobases (kb) encoding a 4.5-kb poly(A)+ RNA restores rhyth-micity when transduced into Drosophila carrying mutations5,6 or chromosomal deletions5 of the per locus and the sequence of this biologically active segment of DNA is reported.
The clock gene period in the housefly, Musca domestica : a molecular analysis
A comparative study suggests that the current model based on the negative feedback loop may be inadequate to explain the molecular mechanism underlying the circadian clock.
Germ-line transformation involving DNA from the period locus in Drosophila melanogaster: overlapping genomic fragments that restore circadian and ultradian rhythmicity to per0 and per- mutants.
P-element-mediated transformations involving DNA fragments from the period (per) clock gene of Drosophila melanogaster have shown that several subsegments of the locus restore rhythmicity to per0 or
Restoration of circadian behavioural rhythms by gene transfer in Drosophila
It is reported that when a 7.1-kb fragment from a per+ fly, including the sequences encoding the 4.5-kb transcript, is introduced into the genome of a per0 (arrhythmic) fly by P element-mediated transformation, circadian rhythmicity of behaviour such as eclosion and locomotor activity is restored.
Changes in abundance or structure of the per gene product can alter periodicity of the Drosophila clock
It is suggested that perl and pers mutants produce hypoactive and hyperactive per proteins, respectively, which are inversely correlated with period length, so that flies with lowest levels of the per product have slow-running biological clocks.
Genetics of circadian clocks
The isolation of circadian clock mutants in Neurospora crassa and Drosophila melanogaster have identified numerous genes whose function is necessary for the normal operation of the circadian clock.
Expression of a Drosophila mRNA is under circadian clock control during pupation.
Pupae that reach the same stage of development at slightly different times of day show a subsequent synchronized rise in 0.9 kb RNA levels, indicating that the expression of this transcript is under circadian clock control.
Mutagenesis of the clock gene period in Drosophila melanogaster
The period gene is an essential component of the circadian oscillator in D.melanogaster and the removal of sequence 3’ to the repeat unexpectedly causes shortening of the period, which suggests that the Thr-Gly deletions may disrupt an interaction with another factor that plays a part in establishing locomotor activity patterns.
In situ localization of the per clock protein during development of Drosophila melanogaster
Per RNA and proteins were detected in a restricted group of cells in the eyes and optic lobes of the adult brain and in many cell bodies in the adult and pupal thoracic ganglia of Drosophila melanogaster.


Molecular genetics of a biological clock in Drosophila.
  • T. Bargiello, M. W. Young
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1984
Physical characterization of a series of chromosomal rearrangements altering per locus activity indicates that DNA affecting behavioral rhythms is found in a 7.1-kb HindIII fragment.
Drosophila maternal and embryo mRNAs transcribed from a single transcription unit use alternate combinations of exons.
The complex transcription pattern of this gene indicates that alternate usage of protein‐coding exons results in the production of different mRNAs with different coding capabilities during oogenesis and embryogenesis.
Neurogenetics of Drosophila Circadian Rhythms
In an effort to understand the genetic control of circadian rhythmicity, as to provide a means of identifying a molecule that may be a component of the underlying oscillator, the laboratory is studying the genetics, physiology, and behavior of Drosophila that bear chemically induced mutations which alter the periodicity of the circadian eclosion and adult locomotor activity rhythms.
Effects of a clock mutation on the subjective day--implications for a membrane model of the Drosophila circadian clock.
The fruit fly brain, like the human brain, possesses an oscillatory system which enables the brain to form a model of the 24 hour cycle in the external environment, and chemically induced mutations affecting circadian rhythmicity were isolated.
The anatomy and function of a segment of the X chromosome of Drosophila melanogaster.
It is postulated that a chromomere is one cistron within which much of the DNA is regulatory in function.
The Isolation of Biological Rhythm Mutations on the Autosomes of Drosophila melanogaster
Like an early-emerging strain previously isolated in Drosophila pseudoobscura thepsi strains, psi-2 brings about an abnormal synchronization of locomotor activity to light-dark cycles and lengthens periods for the activity rhythm in adult emergence.
The isolation of biological rhythm mutations on the autosomes of Drosophila melanogaster.
Like an early-emerging strain previously isolated in Drosophila pseudoobscura, the psi strains have slightly lengthened periods for the circadian rhythm in adult emergence and the emergence of adults in a third mutant strain designated gat is poorly synchronized becoming aperiodic in conditions of continuous darkness and constant temperature.
Nonessential Sequences, Genes, and the Polytene Chromosome Bands of DROSOPHILA MELANOGASTER.
The discovery of these units, which are not allelic to any of the loci previously known, makes it clear that division 3B contains more genes than polytene chromosome bands, while portions of 3A and 3C seem to have no functional significance.