Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger.

@article{Lacalle1989MolecularAO,
  title={Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger.},
  author={Rosa Ana Lacalle and D Pulido and Julia Vara and Magdalena Zalaca{\'i}n and Antonio Jim{\'e}nez},
  journal={Gene},
  year={1989},
  volume={79 2},
  pages={375-80}
}
Nucleotide sequence of a 906-bp fragment of Streptomyces alboniger DNA containing the gene (pac), which encodes a puromycin N-acetyltransferase (PAC), has been determined. The pac gene contains a 600-nt open reading frame, starting with an ATG codon, which encodes a polypeptide of Mr 21,531; this is consistent with the 23 +/- 1.5 kDa size of the PAC enzyme. High-resolution S1 mapping indicates that transcription starts at or next to a C residue 35 bp upstream from the putative ATG start codon… CONTINUE READING

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