Molecular analysis of the lipoglycans of Mycobacterium tuberculosis.

@article{Leopold1993MolecularAO,
  title={Molecular analysis of the lipoglycans of Mycobacterium tuberculosis.},
  author={Klaus Leopold and Werner Fischer},
  journal={Analytical biochemistry},
  year={1993},
  volume={208 1},
  pages={57-64}
}
Lipoglycans were extracted from disrupted cells of Mycobacterium tuberculosis with hot phenol-water. Hydrophobic interaction chromatography on octyl-Sepharose of the crude extract separated nucleic acids and lipoglycans. The latter were retained on the column and fractionated on elution with a propanol gradient into six peaks primarily according to decreasing size of the hydrophilic head groups which dropped from 70 to 5 or 4 monosaccharide residues per molecule. The number of fatty acids per… CONTINUE READING

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