Molecular analysis of the gene encoding an antigenic polypeptide of Trichinella spiralis infective larvae.

@article{Sugane1990MolecularAO,
  title={Molecular analysis of the gene encoding an antigenic polypeptide of Trichinella spiralis infective larvae.},
  author={Kazuo Sugane and Tohru Matsuura},
  journal={Journal of helminthology},
  year={1990},
  volume={64 1},
  pages={1-8}
}
The gene encoding an antigenic polypeptide of Trichinella spiralis infective larvae was studied using recombinant DNA techniques. cDNA synthesized from poly(A)-rich mRNA from T. spiralis infective larvae was ligated into phage vector lambda gt11 DNA and packaged in vitro. The phages were propagated on Escherichia coli and a lambda gt11 expression library was constructed. A cDNA clone encoding a 46 kDa antigenic polypeptide was selected by immunoscreening of the library and identified by the… CONTINUE READING