Molecular analysis of rheumatoid factor (RF)-negative B cell hybridomas from rheumatoid synovial tissue: evidence for an antigen-induced stimulation with selection of high mutated IgVH and low mutated IgVL/lambda genes.

@article{Krenn1999MolecularAO,
  title={Molecular analysis of rheumatoid factor (RF)-negative B cell hybridomas from rheumatoid synovial tissue: evidence for an antigen-induced stimulation with selection of high mutated IgVH and low mutated IgVL/lambda genes.},
  author={Veit Krenn and August K{\"o}nig and Frank Hensel and Claudia Berek and M M Souto Carneiro and Wolfgang Haedicke and Y. Wang and H. Peter Vollmers and H. K. M{\"u}ller-Hermelink},
  journal={Clinical and experimental immunology},
  year={1999},
  volume={115 1},
  pages={168-75}
}
The mutational pattern of IgVH and IgVL genes from synovial tissue B cell hybridomas (n = 8) of patients (n = 4) with rheumatoid arthritis (RA) was analysed, which had been produced by the electrofusion technique without prior in vitro stimulation. The molecular data were correlated with immunohistopathological data and parameters of local disease activity. The IgVH genes of the B cell hybridomas belonged to the VH3 family (DP42; DP47, n = 2; DP53), the VH1 family (DP75), the VH4 family (DP71… CONTINUE READING