Molecular analysis of prokaryotic diversity in the deep subsurface of the former Homestake gold mine, South Dakota, USA

@article{Rastogi2008MolecularAO,
  title={Molecular analysis of prokaryotic diversity in the deep subsurface of the former Homestake gold mine, South Dakota, USA},
  author={Gurdeep Rastogi and Larry D. Stetler and Brent M. Peyton and Rajesh K Sani},
  journal={The Journal of Microbiology},
  year={2008},
  volume={47},
  pages={371-384}
}
A culture-independent molecular phylogenetic analysis was carried out to study the prokaryotic diversity in two soil samples collected from the subsurface (1.34 km depth) of the former Homestake gold mine, Lead, South Dakota, USA at two sites, the Ross shaft and number 6 Winze. Microbial community analyses were performed by cloning and sequencing of 16S rRNA genes retrieved directly from soil samples. Geochemical characterization of soils revealed high amount of toxic metals such as As, Cd, Co… CONTINUE READING
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