Molecular analysis of mouse T cell receptor expression using PCR.

Abstract

This unit describes the use of the polymerase chain reaction (PCR) to characterize rearranged murine T cell receptor (TCR) transcripts present in primary lymphoid tissues, peripheral lymphoid tissues, and extra-lymphoid sites of inflammation, and to quantify them either relatively or absolutely. This methodology has been used extensively to characterize TCR… (More)
DOI: 10.1002/0471142735.im1027s22

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