Molecular analysis of mitotic chromosome condensation using a quantitative time-resolved fluorescence microscopy assay.

@article{Maddox2006MolecularAO,
  title={Molecular analysis of mitotic chromosome condensation using a quantitative time-resolved fluorescence microscopy assay.},
  author={Paul S Maddox and Nathan C Portier and Arshad Desai and Karen Oegema},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2006},
  volume={103 41},
  pages={15097-102}
}
Chromosomes condense during mitotic entry to facilitate their segregation. Condensation is typically assayed in fixed preparations, limiting analysis of contributing factors. Here, we describe a quantitative method to monitor condensation kinetics in living cells expressing GFP fused to a core histone. We demonstrate the utility of this method by using it to analyze the molecular requirements for the condensation of holocentric chromosomes during the first division of the Caenorhabditis elegans… CONTINUE READING

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