Molecular analysis of commensal host-microbial relationships in the intestine.

  title={Molecular analysis of commensal host-microbial relationships in the intestine.},
  author={Lora V. Hooper and Melissa H. Wong and Angelika Thelin and Lennart Hansson and Per G. Falk and Jeffrey I. Gordon},
  volume={291 5505},
Human beings contain complex societies of indigenous microbes, yet little is known about how resident bacteria shape our physiology. We colonized germ-free mice with Bacteroides thetaiotaomicron, a prominent component of the normal mouse and human intestinal microflora. Global intestinal transcriptional responses to colonization were observed with DNA microarrays, and the cellular origins of selected responses were established by laser-capture microdissection. The results reveal that this… 

How host-microbial interactions shape the nutrient environment of the mammalian intestine.

Humans and other mammals are colonized by a vast, complex, and dynamic consortium of microorganisms. One evolutionary driving force for maintaining this metabolically active microbial society is to

Diversity of the Human Intestinal Microbial Flora

A majority of the bacterial sequences corresponded to uncultivated species and novel microorganisms, and significant intersubject variability and differences between stool and mucosa community composition were discovered.

Honor thy symbionts

  • Jian XuJ. Gordon
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2003
Interdisciplinary studies of the effects of the intestinal environment on genome structure and function should provide important new insights about how microbes and humans have coevolved mutually beneficial relationships and new perspectives about the foundations of the authors' health.

Bacteroides thetaiotaomicron in the gut: molecular aspects of their interaction.

Enteric Flora in Health and Disease

It is important to underscore that the specialised lymphoid follicles of the gut mucosa are the major sites for induction and regulation of the immune system.

The Yin and Yang of host-commensal mutualism

It is reported that chronic intestinal inflammation induces commensal E. coli to upregulate stress response genes that paradoxically limit their growth in vivo and the findings are discussed in the context of host-microbial interactions in health and disease and a developing paradigm that may distinguish pathogens from commensals.

Commensal Host-Bacterial Relationships in the Gut

The current genomic revolution offers an unprecedented opportunity to identify the molecular foundations of symbionts and commensals so that the authors can understand how they contribute to their normal physiology and how they can be exploited to develop new therapeutic strategies.

Intestinal microbiota and its functions




A Model of Host-Microbial Interactions in an Open Mammalian Ecosystem

Comparison of conventionally housed and germ-free NMRI mice revealed that production of fucosylated glycoconjugates and an α1,2-fucOSyltransferase messenger RNA in the small-intestinal epithelium requires the normal microflora.

A molecular sensor that allows a gut commensal to control its nutrient foundation in a competitive ecosystem.

A gnotobiotic mouse model is used to show that Bacteroides thetaiotaomicron, a component of the intestinal microflora of mice and humans, uses a repressor, FucR, as a molecular sensor of L-fucose availability.

Creating and Maintaining the Gastrointestinal Ecosystem: What We Know and Need To Know from Gnotobiology

This review focuses on how gnotobiotics—the study of germ-free animals—has been and needs to be used to examine how the gastrointestinal ecosystem is created and maintained.

A primitive T cell-independent mechanism of intestinal mucosal IgA responses to commensal bacteria.

The IgA against intestinal commensal bacterial antigens was analyzed; it was not simply "natural antibody" but was specifically induced and responded to antigenic changes within an established gut flora.

Human fecal flora: the normal flora of 20 Japanese-Hawaiians.

Quantitative and qualitative examination of the fecal flora of 20 clinically healthy Japanese-Hawaiian males was carried out by using anaerobic tube culture techniques, and differential characteristics of previously unreported species are presented.

Initiation of assembly of the cell envelope barrier structure of stratified squamous epithelia.

The data document that CE assembly is initiated along the plasma membrane between desmosomes by head- to-tail and head-to-head cross-linking of involucrin to itself and to envoplakin and perhaps periplakin, and stress the importance of juxtaposition of membranes, transglutaminases, and involUCrin and envoplakia in the initiation of CE assembly of stratified squamous epithelia.

A Selective Medium for Isolation and Presumptive Identification of the Bacteroides fragilis Group

The high selectivity and good plating efficiency of BFAG agar enabled us to isolate the B. fragilis group rapidly from various clinical specimens.

mAngiogenin-3, a target gene of oncoprotein E2a-Pbx1, encodes a new angiogenic member of the angiogenin family.

It is demonstrated that mouse angiogenin-3 is a ribonuclease whose activity and specificity towards tRNA and dinucleotide substrates differ from those of mouse ango-related protein, a non-angiogenic factor, and also induced other molecular events typical of rapidly proliferating endothelial cells, including increases in rough endoplasmic reticulum, polysomes, and mitochondria.

Characterization of mouse angiogenin-related protein: implications for functional studies on angiogenin.

It is found that mouse Ang is potently angiogenic, but Angrp is not, even when assayed at relatively high doses, and it is suggested that the roles of the two proteins in vivo may be quite distinct.