Molecular analysis of bacterial pathogens in otitis media with effusion.

  title={Molecular analysis of bacterial pathogens in otitis media with effusion.},
  author={James Christopher Post and Robert A. Preston and J J Aul and M Larkins-Pettigrew and J Rydquist-White and Kent W. Anderson and Robert M. Wadowsky and Dan Reagan and Elaine S. Walker and Lawrence A. Kingsley and Anthony E Magit and Garth D. Ehrlich},
  volume={273 20},
OBJECTIVE To determine if the polymerase chain reaction (PCR) can detect bacterial DNA in pediatric middle ear effusions that are sterile by standard cultural methods. DESIGN Single-center, blinded, comparative study of diagnostic assays. The PCR-based detection systems for Moraxella catarrhalis, Haemophilus influenzae, and Streptococcus pneumoniae were designed and validated using a battery of DNAs obtained from cultured bacteria. Chronic middle ear effusion specimens were collected and… CONTINUE READING


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