Molecular analysis of a metalloprotease from Proteus mirabilis.

  title={Molecular analysis of a metalloprotease from Proteus mirabilis.},
  author={Chris Wassif and Donald B Cheek and Robert Belas},
  journal={Journal of bacteriology},
  volume={177 20},
Proteus mirabilis is known for its ability to differentiate from swimmer to swarmer cells, a process crucial for the pathogenesis of these bacteria during urinary tract infections. Among the many virulence factors produced during swarmer cell differentiation is an extracellular metalloprotease. A cosmid containing a large fragment of P. mirabilis chromosomal DNA was obtained by measuring protease expression in recombinant Escherichia coli. The recombinant and native enzymes were purified to… CONTINUE READING
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