Molecular analysis of a carbohydrate antigen involved in the structure and function of zona pellucida glycoproteins.

@article{Dunbar2001MolecularAO,
  title={Molecular analysis of a carbohydrate antigen involved in the structure and function of zona pellucida glycoproteins.},
  author={Bonnie S. Dunbar and Terry Kramer Timmons and Stephanie M Skinner and Sateesh. P Prasad},
  journal={Biology of reproduction},
  year={2001},
  volume={65 3},
  pages={
          951-60
        }
}
A lactosaminoglycan-associated antigen is associated with a carbohydrate moiety of all three zona pellucida (ZP) glycoproteins of pig and rabbit but is absent in the mouse and rat. A monoclonal antibody (PS1) recognizing this determinant was obtained by immunizing mice with a porcine ZP glycoprotein isoform purified by two-dimensional polyacrylamide gel electrophoresis. Conditions known to remove O-linked or sialic acid carbohydrate moieties (alkaline reduction; O-glycanase or neuraminidase… CONTINUE READING
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