Molecular Techniques for Diagnosis of Bacterial Plant Pathogens

  title={Molecular Techniques for Diagnosis of Bacterial Plant Pathogens},
  author={Sakshi R Tewari and Shilpi Sharma},
  journal={Microbial Diversity in the Genomic Era},

Comprehensive account of present techniques for in-field plant disease diagnosis

The aim of this review is to compile the information on current promising techniques that are in use for plant-pathogen diagnosis and focuses on the latest in-field pathogen diagnostic techniques with associated advantages and limitations.

Rapid genotyping of bacterial leaf blight resistant genes of rice using loop-mediated isothermal amplification assay

The LAMP assay developed in this study provides a simple, specific, sensitive, robust, and cost-effective method for foreground selection of R genes in the resistance breeding programs of resource-poor laboratory.

LAMP-based foldable microdevice platform for the rapid detection of Magnaporthe oryzae and Sarocladium oryzae in rice seed

This is the first study to report a LAMP-based foldable microdevice platform to detect any plant pathogens, and could serve as a prototype for developing on-field diagnostic kits to be used at the point of care centers for the rapid diagnosis of M. oryzae and S.oryzae in rice seeds.

Plant carotenoids evolution during cultivation, postharvest storage, and food processing: A review.

In this article, evolution of carotenoids during cultivation, postharvest storage, and food processing is comprehensively reviewed and Appropriate conditions and methods to cultivate, store, and process fruit and vegetables to help retardCarotenoid degradation and enhance carOTenoid biosynthesis are identified.

Molecular Tools and Techniques for Detection and Diagnosis of Plant Pathogens

The development in the recent tools in molecular biology has enhanced and accelerated the detection and diagnostics through the automatic purification of nucleic acids and specific proteins from pathogens.

Integrated approaches for detection of plant pathogenic bacteria and diagnosis of bacterial diseases.

  • A. M. Alvarez
  • Biology, Medicine
    Annual review of phytopathology
  • 2004
This review focuses on rapid immunodiagnostic and DNA-based detection methods for known bacterial pathogens in plants or plant products, which often manifest no symptoms of disease.

Plant pathogen nanodiagnostic techniques: forthcoming changes?

Nanotechnology, nano particles and quantum dots have emerged as essential tools for fast detection of a particular biological marker with extreme accuracy, allowing experts to help farmers in the prevention of epidemic diseases.

Current and Prospective Methods for Plant Disease Detection

The review provides a comprehensive overview of biosensors based on highly selective bio-recognition elements such as enzyme, antibody, DNA/RNA and bacteriophage as a new tool for the early identification of crop diseases.

Innovative tools for detection of plant pathogenic viruses and bacteria

The advances in research that will result from the sequencing of many plant pathogen genomes represent a new source of information for the future development of sensitive and specific detection techniques for these microorganisms.

Diagnosis and Management of Bacterial Plant Diseases

For an effective disease management, the correct diagnosis of a disease based on the identification of its causal agent is very essential and a comprehensive host list that covers a known disease, its typical symptoms and its known potential pathogens for a specific host is required.

Molecular detection of plant pathogenic bacteria using polymerase chain reaction single-strand conformation polymorphism.

The potential use of PCR-SSCP technique for the detection and diagnosis of phytobacterial pathogens is discussed in the present paper.

First detection of Pseudomonas viridiflava, the causal agent of blossom blight in apple by using specific designed primers

This study conducted for detection and identification of the causal agent of blossom blight of apple, which results in blast of whole tree, identified as Pseudomonas viridiflava based on newly designed consensus primer pair (PsV-F and PsV-R).