Molecular Properties, Functional Mechanisms, and Applications of Sliced siRNA

@inproceedings{Sun2015MolecularPF,
  title={Molecular Properties, Functional Mechanisms, and Applications of Sliced siRNA},
  author={Guihua Sun and Spencer Yele Yeh and Christine Wan-Yin Yuan and Margaret Jean-Yih Chiu and Bryan Shing Hei Yung and Yun Yen},
  booktitle={Molecular therapy. Nucleic acids},
  year={2015}
}
Using pre-miR-451 as a model molecule, we have characterized the general molecular properties of small hairpin RNAs that are processed into potent small interfering RNAs (siRNA) by Argonaute2 (Ago2). The Ago2-sliced siRNAs (sli-siRNAs) have the same silencing potency as the classical Dicer diced siRNAs (di-siRNAs) but have dramatically reduced unwanted sense strand activities. We have built vectors with the constitutive or inducible U6 promoter that can express sli-siRNAs in mammalian cells, in… CONTINUE READING