Molecular Phylogeny of the Genus Dactylopius (Hemiptera: Dactylopiidae) and Identification of the Symbiotic Bacteria

  title={Molecular Phylogeny of the Genus Dactylopius (Hemiptera: Dactylopiidae) and Identification of the Symbiotic Bacteria},
  author={S. Tabita Ram{\'i}rez-Puebla and M{\'o}nica Rosenblueth and C. K. Ch{\'a}vez-Moreno and M. C. Catanho Pereira De Lyra and Alberto Tecante and Esperanza Mart{\'i}nez-Romero},
  booktitle={Environmental entomology},
ABSTRACT Phylogenetic analyses, from polymerase chain reaction (PCR)-amplified 12S rRNA and 18S rRNAgene sequences from cochineal insects of the genus Dactylopius present in Mexico, showed that D. ceylonicus, D. confusus, and D. opuntiae are closely related. D. coccus constitutes a separate clade, and D. tomentosus is the most distantly related. Bacterial 16S rRNA sequences from all the Dactylopius species sampled showed a common &bgr;-proteobacteria, related to Azoarcus, also found in eggs… 

Fungal Community Associated with Dactylopius (Hemiptera: Coccoidea: Dactylopiidae) and Its Role in Uric Acid Metabolism

The presence of fungal genes involved in UA catabolism is confirmed, suggesting that fungi help in the nitrogen recycling process in Dactylopius by uricolysis.

Higher classification of mealybugs (Hemiptera: Coccomorpha) inferred from molecular phylogeny and their endosymbionts

Based on the data from DNA sequences, morphology and obligate endosymbionts, a phylogeny supporting the families Rhizoecidae and Xenococcidae separate from Pseudococc Families is presented, and the separation of Rastrococcus Ferris from Phenacoccinae and PseudOCoccinee is excluded.

Evolutionary relationships of flavobacterial and enterobacterial endosymbionts with their scale insect hosts (Hemiptera: Coccoidea)

The authors' analyses suggest that Enterobacteriaceae underwent more evolutionary events (losses, duplications and host switches), and their phylogenies showed a lower proportion of congruent nodes between host and bacteria, indicating a more relaxed relationship with scale insects compared with Flavobacteria.

Metatranscriptomic Analysis of the Bacterial Symbiont Dactylopiibacterium carminicum from the Carmine Cochineal Dactylopius coccus (Hemiptera: Coccoidea: Dactylopiidae)

In most insects, nitrogen-fixing bacteria are found in the gut, but in this study they are shown to occur in the hemolymph, probably delivering essential amino acids and riboflavin to the host from nitrogen substrates derived from nitrogen fixation.

Candidatus Dactylopiibacterium carminicum, a Nitrogen-Fixing Symbiont of Dactylopius Cochineal Insects (Hemiptera: Coccoidea: Dactylopiidae)

Dactylopiibacterium symbionts may compensate for the nitrogen deficiency in the cochineal diet, and may provide essential amino acids, recycle uric acid, and increase the coChineal life span.

Coexistence of novel gammaproteobacterial and Arsenophonus symbionts in the scale insect Greenisca brachypodii (Hemiptera, Coccomorpha: Eriococcidae)

These findings provide evidence for a polyphyletic origin of symbionts of Eriococcidae, and propose the name 'Candidatus Kotejella greeniscae' for its tentative classification.

Genetic variation amongst biotypes of Dactylopius tomentosus

This study is the first molecular characterization of cochineal biotypes that, together with Wolbachia sequences, contribute to the better identification of the biotypes of co chineal insects and to the biological control of cacti using host‐specific biotype of the scale.

Genetic variability of Dactylopius opuntiae (Hemiptera, Dactylopiidae) on forage cactus in northeast Brazil.

The low genetic variability demonstrates a high degree of relatedness among these D. opuntiae populations, and underpins morphological characterization and supports the monitoring of populations in integrated management programs designed to control this pest.

Range Wide Phylogeography of Dactylopius coccus (Hemiptera: Dactylopiidae)

The center of origin of D. coccus is found to be Oaxaca Mexico based on mtDNA data and climate niche modeling and further meta-genomic data are needed to rule out selective sweeps from past and present endosymbionts.



A preliminary phylogeny of the scale insects (Hemiptera: Sternorrhyncha: Coccoidea) based on nuclear small-subunit ribosomal DNA.

The Opuntia (Cactaceae) and Dactylopius (Hemiptera: Dactylopiidae) in Mexico: a historical perspective of use, interaction and distribution

Strategies for in situ conservation combined with re-established use and cochineal production may enhance conservation policies of Dactylopius and Opuntia species.

Molecular phylogeny of the homoptera: a paraphyletic taxon

Molecular data corroborate current views of relationships within the Sternorrhyncha and Auchenor rhyncha based on morphology and strongly support the hypothesis of homopteran paraphyly as stated above.

The biology of Dactylopius tomentosus (Hemiptera: Dactylopiidae)

Investigating the life cycle and the morphological and reproductive characteristics of D. tomentosus revealed unique characteristics of this economically important species, underlines the need to study each species in this genus.

Uliginosibacterium gangwonense gen. nov., sp. nov., isolated from a wetland, Yongneup, in Korea.

The results of phenotypic, chemotaxonomic and phylogenetic analyses indicate that strain 5YN10-9(T) represents a novel species of a new genus within the family Rhodocyclaceae, class Betaproteobacteria, for which the name Uliginosibacterium gangwonense gen. nov. is proposed.

Secondary (γ-Proteobacteria) Endosymbionts Infect the Primary (β-Proteobacteria) Endosymbionts of Mealybugs Multiple Times and Coevolve with Their Hosts

The results of phylogenetic analyses of mitochondrial DNA fragments encoding cytochrome oxidase subunits I and II from four representative mealybugs species were in agreement with the results of 16S-23S rDNA analyses, suggesting that relationships among strains of “Candidatus T. princeps” are useful in inferring the phylogeny of their mealybug hosts.

Organization of the mitochondrial genomes of whiteflies, aphids, and psyllids (Hemiptera, Sternorrhyncha)

Two species of whiteflies, one psyllid and one aphid have mitochondrial genomes with a gene order very similar to that of the proposed insect ancestor, indicating a transposition in the ancestor of these clusters.

Presence of Wolbachia in Insect Eggs Containing Antimicrobially Active Anthraquinones

The analyses revealed that a representative of the Galerucini, Galeruca tanaceti and Dactylopius, are indeed infected by endosymbiotic Wolbachia bacteria, and the presence of antimicrobial compounds such as anthraquinones does not necessarily protect insects from infection by WolbachIA.

Evolution and diversity of Rickettsia bacteria

It is shown that Rickettsia are primarily arthropod-associated bacteria, and several novel groups within the genus are identified, and multi-gene analysis indicates that different parts of the genome tend to share the same phylogeny.