Molecular Monitoring Neural Activity and [ Ca 2 ] with Genetically Encoded Ca 2 Indicators

@inproceedings{Pologruto2004MolecularMN,
  title={Molecular Monitoring Neural Activity and [ Ca 2 ] with Genetically Encoded Ca 2 Indicators},
  author={Thomas A Pologruto and Ryohei Yasuda and Karel Svoboda},
  year={2004}
}
Genetically encoded Ca 2 indicators (GECIs) based on fluorescent proteins (XFPs) and Ca 2 -binding proteins [like calmodulin (CaM) ] have great potential for the study of subcellular Ca 2 signaling and for monitoring activity in populations of neurons. However, interpreting GECI fluorescence in terms of neural activity and cytoplasmic-free Ca 2 concentration ([Ca 2 ]) is complicated by the nonlinear interactions between Ca 2 binding and GECI fluorescence. We have characterized GECIs in… CONTINUE READING

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