Molecular Mechanisms of Gene Silencing Mediated by

  title={Molecular Mechanisms of Gene Silencing Mediated by},
  author={Michela Curradi and Annalisa Izzo and Gianfranco Badaracco and Nicoletta Landsberger},
DNA methylation and chromatin modification operate along a common pathway to repress transcription; accordingly, several experiments demonstrate that the effects of DNA methylation can spread in cis and do not require promoter modification. In order to investigate the molecular details of the inhibitory effect of methylation, we microinjected into Xenopus oocytes a series of constructs containing a human CpG-rich sequence which has been differentially methylated and cloned at different… CONTINUE READING