Molecular Machines : Putting the Pieces Together

  title={Molecular Machines : Putting the Pieces Together},
  author={Eva Nogales and Nikolaus Grigorieff},
The use of EM and image analysis techniques, particularly the single particle methodology, has opened the door to the structural characterization of large protein complexes in the megadalton range. This review covers the basic principles of single particle EM and recent examples of its application to the study of large molecular machines in the eukaryotic cell. These examples will give the reader a perspective on how this emerging technique can be applied to the study of large protein complexes… CONTINUE READING