Molecular Diversity of Tuliposide A-Converting Enzyme in the Tulip

  title={Molecular Diversity of Tuliposide A-Converting Enzyme in the Tulip},
  author={Taiji Nomura and Aya Tsuchigami and Shinjiro Ogita and Yasuo Kato},
  journal={Bioscience, Biotechnology, and Biochemistry},
  pages={1042 - 1048}
Tuliposide A-converting enzyme (TCEA) catalyzes the conversion of 6-tuliposide A to its lactonized aglycon, tulipalin A, in the tulip (Tulipa gesneriana). The TgTCEA gene, isolated previously from petals, was transcribed in all tulip tissues but not in the bulbs despite the presence of TCEA activity, which allowed prediction of the presence of a TgTCEA isozyme gene preferentially expressed in the bulbs. Here, the TgTCEA-b gene, the TgTCEA homolog, was identified in bulbs. TgTCEA-b polypeptides… 

Molecular diversity of tuliposide B-converting enzyme in tulip (Tulipa gesneriana): identification of the root-specific isozyme

Tissue-specific isozymes are involved in the conversion of 6-tuliposide B, a major secondary metabolite in tulip, to antibacterial tulipalin B, which encodes a TCEB.

Molecular diversity of tuliposide B-converting enzyme in tulip (Tulipa gesneriana): identification of the third isozyme with a distinct expression profile

Identification of TgTCEB-L explains the PosB-converting activity detected in tissues where T gTCEB1 and Tg TCEB-R transcripts could not be detected, indicating that tulip subtilizes the three TGTCEB isozymes depending on the tissue.

Molecular identification of tuliposide B-converting enzyme: a lactone-forming carboxylesterase from the pollen of tulip.

Results indicate that mature TgTCEB1 is specifically localized in plastids of pollen grains, but not in the intracellular spaces of pollen grain, as previously reported.

One-Step Enzymatic Synthesis of 1-Tuliposide A Using Tuliposide-Converting Enzyme

By using the TCEs, this work established a facile enzymatic process for 1-PosA synthesis from the naturally occurring 1,6-diacyl-glucose type of Pos (PosD and PosF), and discovered that TCEA and TCEB react preferentially with PosD andPosF, respectively, to form 1- PosA and the corresponding Pa derived from the 6-acyl group.

Identification of tuliposide G, a novel glucoside ester-type tuliposide, and its distribution in tulip

  • T. NomuraY. Kato
  • Biology
    Zeitschrift fur Naturforschung. C, Journal of biosciences
  • 2020
Quantification of PosG in bulbs of 52 types of tulip, including 30 cultivars and 22 wild Tulipa spp.

Function and application of a non-ester-hydrolyzing carboxylesterase discovered in tulip

  • T. Nomura
  • Biology
    Bioscience, biotechnology, and biochemistry
  • 2017
This review describes the biochemical and physiological aspects of tulipalin biogenesis, and the diverse functions of plant carboxylesterases in the α/β-hydrolase fold superfamily.

Isolation and identification of tuliposides D and F from tulip cultivars

This is the first report that substantiates the presence of 1,6-diacyl-glucose type of Pos in tulip cultivars.

Environmentally benign process for the preparation of antimicrobial α-methylene-β-hydroxy-γ-butyrolactone (tulipalin B) from tulip biomass

An environmentally benign and facile process for the preparation of PaB using tulip biomass materials based on one-step enzyme reaction catalyzed by TCE without the use of petroleum-derived solvents is described.



A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip1[W]

To the knowledge, TgTCEA is the first identified member of the lactone-forming carboxylesterases, specifically catalyzing intramolecular transesterification in the tulip defensive strategy.

Purification and Characterization of a Tuliposide-Converting Enzyme from Bulbs of Tulipa gesneriana

An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana and had maximum activity and stability at neutral pH and moderate temperature.

Isolation and quantification of tuliposides and tulipalins in tulips (Tulipa) by high‐performance liquid chromatography

6‐Tuliposide A and tulipalin A seem to be the major allergens in tulips, although tuliposides D and F may also contribute to the allergenic properties.

Asymmetric Total Synthesis of 6-Tuliposide B and Its Biological Activities against Tulip Pathogenic Fungi

Both the tuliposides and tulipalins showed pigment-inducing activity against Gibberella zeae and inhibitory activity against Fusarium oxysporum f.

High-resolution crystal structure of plant carboxylesterase AeCXE1, from Actinidia eriantha, and its complex with a high-affinity inhibitor paraoxon.

The structural information for AeCXE1 provides a basis for addressing the wider functional roles of carboxylesterases in plants, and is most closely related structurally to the hormone-sensitive lipase subgroup.

A fungitoxic substance extracted from tulips and its possible role as a protectant against disease

It was concluded that the fungus is not able to penetrate into the tulip bulb during the greater part of the growing season, and a protecting barrier was demonstrated to be present in the white skin.