Molecular Differentiation of Shigella Spp. from Enteroinvasive E. Coli

@article{Lbersli2016MolecularDO,
  title={Molecular Differentiation of Shigella Spp. from Enteroinvasive E. Coli},
  author={Inger L{\o}bersli and A. L. Wester and Anders Kristiansen and Lin T. Brandal},
  journal={European Journal of Microbiology \& Immunology},
  year={2016},
  volume={6},
  pages={197 - 205}
}
Key MethodA real-time polymerase chain reaction (PCR) assay, amplifying the genes encoding lactose permease (lacY) and invasion plasmid antigen H (ipaH), was run on 121 isolates phenotypically classified as Shigella spp., enteroinvasive Escherichia coli (EIEC), or EIEC O nontypable (ONT). The results were compared with data from a generic E. coli multiple-locus variable-number of tandem repeat analysis (MLVA) and a Shigella MLVA. The real-time PCR verified all Shigella spp. (n = 53) as Shigella (lacY…Expand
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