Molecular Detection of 16SrXI Group Phytoplasma Associated with Root (Wilt) Disease of Coconut (Cocos nucifera) in India.

  title={Molecular Detection of 16SrXI Group Phytoplasma Associated with Root (Wilt) Disease of Coconut (Cocos nucifera) in India.},
  author={R. Manimekalai and V. P. Soumya and R. Sathish Kumar and Ramasamy Selvarajan and Krishna M. Reddy and George V. Thomas and M. Sasikala and Gupta Rajeev and Vikas Baranwal},
  journal={Plant disease},
  volume={94 5},
Coconut palm (Cocos nucifera L.), a versatile tree crop with multifarious uses, is important for the livelihood security of millions of people in India. Root (wilt) disease (RWD) is a major production constraint causing an estimated yield loss of 968 million nuts in southern India. Affected palms show bending of leaflets (flaccidity), foliar yellowing, and marginal necrosis. Phytoplasmas have been observed to be associated with this disease by electron microscopy (EM) and transmission (3) but… 

A phytoplasma is associated with the Weligama coconut leaf wilt disease in Sri Lanka.

The possible phytoplasma aetiology of Weligama coconut leaf wilt disease was investigated and it was confirmed that a phy toplasma belonging to the 16SrXI ‘Candidatus Phy Toplasma oryzae’ group is associated with WCLWD.

Identification and Characterization of 16SrIX and 16SrXI groups of phytoplasmas associated with leaf yellows and declining disease of garlic and onion in India.

This is the first report of association of 16SrXI and -IX groups of phytoplasma causing leaf yellows and declining disease of onion and garlic in India.

First report of occurrence of Candidatus Phytoplasma trifolii-related strain causing witches’ broom disease of chilli in India

The chilli witches’ broom phy toplasma (CWBP) sequence shared 100% nucleotide sequence identity with the 16S rDNA sequences of identified strains of ‘Candidatus Phytoplasma trifolii’ subgroup D (16SrVI-D) phytoplasmas associated with Lens culinaris’ witches'-broom.

Molecular characterization of ‘Clover proliferation’ phytoplasma subgroup-D (16SrVI-D) associated with vegetables crops in India

Nine vegetable plants species exhibiting phytoplasma suspected symptoms of white/purple leaf, little leaf, flat stem, witches’ broom, phyllody and leaf yellowing were observed in experimental fields at Indian Agricultural Research Institute, New Delhi from December 2015 to July 2016 and identification of 16SrVI subgroup D strain of phy toplasmas in four vegetables and one leafhopper species was confirmed.

Molecular characterization of phytoplasmas of ‘Clover proliferation’ group associated with three ornamental plant species in India

Phylogenetic and virtual RFLP analysis of 16S rDNA sequences of the five identified phytoplasma strains belonging to three ornamental species further confirmed their clustering and grouping with member strains of ‘clover proliferation’ subgroup D.

Evidence of 16SrXI group phytoplasma DNA in embryos of root wilt diseased coconut palms

To check the potential seed transmission of phytoplasma, mature nuts from diseased palms were germinated in poly bags under disease free conditions and seedlings were sampled for DNA isolation and nested PCR analysis, but phy toplasma DNA could not be detected in any of the seedlings raised inpoly bags.

Identification and characterization of Candidatus Phytoplasma trifolii (16SrVI-D) inducing shoot proliferation disease of potato in India

Potato is confirmed as a new host of 16SrVI-D subgroup of phytoplasmas in the world and poses serious socio-economic threat to commercially grown potato crops.

Detection and characterization of 16SrI-B phytoplasmas associated with yellow leaf disease of arecanut palm in India

Real and virtual RFLP analysis suggested that the arecanut phytoplasmas in the present study belongs to 16SrI-B subgroup, the first report from India on yellow leaf disease of are canut.

Characterization of phytoplasmas associated with sesame (Sesamum indicum) phyllody disease in North India utilizing multilocus genes and RFLP analysis

The BLAST analysis and phylogenetic analysis of 16Sr RNA and secA gene sequences revealed that phy toplasma associated with SP and WB isolates in all the three states belonged to 'Candidatus Phytoplasma asteris' (16SrI) and 'Candidia aurantifolia', however, tuf and groEL gene specific primers could not distinguish SP andWB isolates into different group level status.