• Corpus ID: 85959433

Molecular Cloning andGenetic Organization ofC4-Dicarboxylate Transport GenesfromRhizobium leguminosarum

  title={Molecular Cloning andGenetic Organization ofC4-Dicarboxylate Transport GenesfromRhizobium leguminosarum},
  author={Clive W. Ronson},
Cosmids containing C4-dicarboxylate transport (dct) geneswere identified froma genebankofRhizobium leguminosarum DNA madeinthebroad-host-range vector pLAFRIbytheir ability tocomplement R.trifolii det mutants. Thedct geneswere further characterized bysubcloning, restriction site mapping, andtransposon TnS andTn7mutageneses. Threedctloci were identified within a 5.5-kilobase region ofDNA,intheorderdctAdctB-dctC. Theresults suggested thatdctAencoded a structural componentnecessaryforC4… 
8 Citations

Mutants ofRhizobium meliloti Defective inSuccinate Metabolism

It was found that infree-lvlng cells, Pck is required forthesynthesis ofphoenopyruvate during b, and addititheenzyms of the lower half of theEmbden-Meyerhoff-Parnas pathway wereabsolutely required for forglu Iss.

Alfalfa yield response to inoculation with recombinant strains of Rhizobium meliloti with an extra copy of dctABD and/or modified nifA expression

It is concluded that RMBPC -2 can increase alfalfa yields under field conditions of nitrogen limitation, low endogenous rhizobial competitors, and sufficient moisture.

Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

It is shown that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation and may be constitutively expressed.

The ntrA gene of Agrobacterium tumefaciens: identification, cloning, and phenotype of a site-directed mutant

A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrC gene of Rhizobium meliloti as a probe to demonstrate the requirement of the nTrA gene for nitrate utilization and C4-dicarboxylate transport but not for vir gene expression or tumorigenesis.

Two succinate uptake systems inBradyrhizobium japonicum

Succinate uptake inBradyrhizobium japonicum 61-A-101 has a biphasic kinetic, indicating the presence of a high-and low-affinity uptake system, and in Enterobacter aerogenes NCTC 10006, only one uptake system for succinate was found.

Conservation between coding and regulatory elements of Rhizobium meliloti and Rhizobium leguminosarum dct genes

Complementation of Rhizobium leguminosarum dct mutants with a cosmid bank yielded Rhizobium meliloti homologs of the dctA, dctB, and dctD genes. The genes dctB and dctD are thought to form a



Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

P15A-derived plasmids were not self-transmissible and were mobilized poorly by Hfr strains; however, mobilization was complemented by the presence of a ColE1 plasmid within the same cell.

Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

  • D. ClewellD. Helinski
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1969
Electron microscopic analyses indicate that the decrease in sedimentation rate of the ColE(1)-protein complex after treatment with these various agents is largely owing to an induced transition of ColE (1) DNA from the supercoiled to the open circular state.

Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA‐like gene

The region of the Rhizobium leguminosarum plasmid pRL1JI involved in nodulation and nitrogen fixation has been cloned on a series of four overlapping cosmid clones and DNA homologous to the nifA gene of Klebsiella pneumoniae has been identified.

Regulation: positive control.