Molecular Cloning and Characterization of a Channel-like Transporter Mediating Intestinal Calcium Absorption*

@article{Peng1999MolecularCA,
  title={Molecular Cloning and Characterization of a Channel-like Transporter Mediating Intestinal Calcium Absorption*},
  author={J B Peng and Xing Chen and Urs V. Berger and Peter M. Vassilev and Hiroyasu Tsukaguchi and Edward Meigs Brown and M. A. Hediger},
  journal={The Journal of Biological Chemistry},
  year={1999},
  volume={274},
  pages={22739 - 22746}
}
Calcium is a major component of the mineral phase of bone and serves as a key intracellular second messenger. Postnatally, all bodily calcium must be absorbed from the diet through the intestine. Here we report the properties of a calcium transport protein (CaT1) cloned from rat duodenum using an expression cloning strategy in Xenopus laevis oocytes, which likely plays a key role in the intestinal uptake of calcium. CaT1 shows homology (75% amino acid sequence identity) to the apical calcium… Expand
Human calcium transport protein CaT1.
TLDR
A role for hCaT1 in cellular calcium uptake in a variety of tissues, including the transcellular calcium transport pathway in intestine, is suggested. Expand
Epithelial calcium transporter expression in human duodenum.
TLDR
Although individual variations in calcium channel transcripts are not vitamin D dependent, expression of genes governing apical entry and basolateral extrusion are tightly linked, which may account for some of the unexplained variability in calcium absorption. Expand
Characterization of the Human Intestinal Calcium Transporter, CaT1, Stably Expressed in CHO Cells
The human calcium transporter, hCaT1, was cloned and analyzed. The obtained amino acid sequence was slightly different from the ortholog of hCaT1 which had been identified by Peng et al. (2000.Expand
Molecular Mechanisms of Intestinal Transport of Calcium, Phosphate, and Magnesium
Abstract Many new insights into the molecular mechanisms of intestinal absorption of calcium, phosphate, and magnesium have been made over the past decade. These include the identification, cloning,Expand
Characterization of the Human Intestinal Calcium Transporter, CaT1, Stably Expressed in CHO Cells.
The human calcium transporter, hCaT1, was cloned and analyzed. The obtained amino acid sequence was slightly different from the ortholog of hCaT1 which had been identified by Peng et al. (2000.Expand
Estrogen, Vitamin D, and Calcium Transport
  • S. Christakos, R. Prince
  • Medicine
  • Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
  • 2003
TLDR
The study of Van Cromphaut et al. suggests that estrogen and hormonal changes during pregnancy or lactation can regulate mechanisms involved in the calcium influx process in the intestine independent of 1,25(OH)2D3, and suggests both independent and coordinate regulation of intestinal CaT1 and calbindin. Expand
Calcium-Selective Ion Channel, CaT1, Is Apically Localized in Gastrointestinal Tract Epithelia and Is Aberrantly Expressed in Human Malignancies
TLDR
It is established for the first time that CaT1 localizes to the apical membrane of intestinal absorptive cells, thereby providing the first direct evidence that this protein is in fact an apical entry channel in the gastrointestinal tract. Expand
Expression patterns of intestinal calcium transport factors and ex-vivo absorption of calcium in horses
TLDR
The present findings suggest that TRPV6, CB and VDR may be involved in active intestinal Ca2+ absorption in horses, as described for other mammals. Expand
Molecular cloning, tissue distribution, and chromosomal mapping of the human epithelial Ca2+ channel (ECAC1).
Functional and morphological analyses indicated that the epithelial Ca2+ channel (ECaC), which was recently cloned from rabbit kidney, exhibits the defining properties for being the gatekeeper inExpand
Gene structure and chromosomal mapping of human epithelial calcium channel.
TLDR
The structure of the human ECaC gene, which represents the rate-limiting step of vitamin D(3)-regulated Ca(2+) (re)absorption in kidney and intestine, is reported here to supply the basis for direct mutation analysis. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 46 REFERENCES
Molecular Identification of the Apical Ca2+Channel in 1,25-Dihydroxyvitamin D3-responsive Epithelia*
TLDR
This novel epithelial Ca2+ channel (ECaC) of 730 amino acids contains six putative membrane-spanning domains with an additional hydrophobic stretch predicted to be the pore region and has the expected properties for being the gatekeeper of 1,25-dihydroxyvitamin D3-dependent active transepithelial Ca 2+ transport. Expand
Characterization of a Rat Na+-Dicarboxylate Cotransporter*
TLDR
The cloning and characterization of a rat kidney dicarboxylate transporter (SDCT1) is reported and new insights are provided into the biophysical characteristics and physiological implications of a cloned dicARboxylates transporter. Expand
Calcium transport mechanism in human colonic apical membrane vesicles.
TLDR
The uptake of Ca2+ by human colonic apical membranes involves predominantly a carrier-mediated transport mechanism that is unaffected by outwardly directed H+, K+, and Na+ gradients. Expand
Intestinal calcium transport and calcium extrusion processes at the basolateral membrane.
TLDR
Attention in this report is given to the transcellular models of Ca2+ absorption and, more specifically, theCa2+ extrusion events occurring at the basolateral membrane, which include the operation of an ATP-dependent Ca2-dependent pump and a Na+/Ca2- exchanger. Expand
Segmental heterogeneity of cellular and paracellular calcium transport across the rat duodenum and jejunum.
TLDR
Tissue resistance is decreased and the flux of the paracellular marker mannitol in both directions is increased, suggesting that 1,25(OH)2D3 stimulates diffusive calcium flux across both segments by increasing theParacellular permeability. Expand
Identification of a Drosophila gene encoding a calmodulin-binding protein with homology to the trp phototransduction gene
TLDR
The identification of a protein similar to thetrp gene product, yet also able to bind Ca2+/calmodulin, allows for a reinterpretation of the phenotype of the trp mutations and suggests that both genes may encode light-sensitive ion channels. Expand
Characterization of calcium uptake by brush border membrane vesicles of human small intestine.
TLDR
The duodenum appears to have a high-affinity, high-capacity system for the transport of calcium in humans, and these studies are the first to characterize calcium transport by brush border membranes of the human small intestine. Expand
Vitamin D and intestinal calcium transport: facts, speculations and hypotheses.
TLDR
This review emphasizes some aspects of the Ca2+ absorptive process that require resolution and/or further experimental support and discusses the vitamin D-dependency of the paracellular transfer of Ca2+, which is affected by vitamin D status. Expand
Calcium transport across epithelia.
  • F. Bronner
  • Biology, Medicine
  • International review of cytology
  • 1991
TLDR
This chapter discusses mechanisms by which cells transport calcium and modify calcium fluxes in the body and using radioactive or stable isotopes of calcium, along with a balance procedure, one of the streams of calcium in the intestine can be labeled and the true amount absorbed and the quantity of endogenous calcium lost in the stool can be quantitated. Expand
Vitamin D3-Induced Calcium-Binding Protein in Chick Intestinal Mucosa
TLDR
The vitamin D3-enhanced duodenal absorption of Ca47 in rachitic chicks occurred almost simultaneously with the appearance of the vitamin D 3-induced factor, and there was good correlation between the concentration of binding factor and the rate of absorption ofCa47. Expand
...
1
2
3
4
5
...