Molecular Cloning and Characterization of Tyrosine Aminotransferase and Hydroxyphenylpyruvate Reductase, and Rosmarinic Acid Accumulation in Scutellaria baicalensis

@article{Kim2014MolecularCA,
  title={Molecular Cloning and Characterization of Tyrosine Aminotransferase and Hydroxyphenylpyruvate Reductase, and Rosmarinic Acid Accumulation in Scutellaria baicalensis},
  author={Yeon-Bok Kim and Md. Romij Uddin and Yeji Kim and Chun-Geon Park and Sang Un Park},
  journal={Natural Product Communications},
  year={2014},
  volume={9}
}
Rosmarinic acid (α-O-caffeoyl-3,4-dihydroxyphenylacetic acid, RA) is a caffeoyl ester widely distributed in plants. cDNA clones encoding tyrosine aminotransferase (TAT1 and 2) and hydroxyphenylpyruvate reductase (HPPR) have been isolated from Scutellaria baicalensis. The open reading frames (ORFs) of SbTAT1 and 2 were 1230 and 1272 bp long and encoded 409 and 423 amino acid residues, respectively. HPPR corresponded to a 942-bp ORF and 313 amino acid residues of translated protein. To study the… 

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References

SHOWING 1-10 OF 30 REFERENCES
Purification, Cloning and Functional Expression of hydroxyphenylpyruvate Reductase Involved in Rosmarinic Acid Biosynthesis in Cell Cultures of Coleus Blumei
TLDR
The sequence showed that HPPR belongs to the family of D-isomer-specific 2-hydroxyacid dehydrogenases, and the protein was shown to catalyse the NAD(P)H-dependent reduction of 4-hydroxyphenylpyruvate to 4-Hydroxylapatite chromatography, and 3,4-dihydroxyp Henyllactate to 3- dihydroxyyphenyllactates.
Characterization and expression profiling of tyrosine aminotransferase gene from Salvia miltiorrhiza (Dan-shen) in rosmarinic acid biosynthesis pathway
TLDR
expression analysis reveals that the signaling components of defense/stress pathways, such as methyl jasmonate (MeJA), abscisic acid (ABA), salicylic acid (SA) and ultraviolet-B radiation (UV-B), up-regulate the SmTAT transcript levels over the control.
Accumulation of tilianin and rosmarinic acid and expression of phenylpropanoid biosynthetic genes in Agastache rugosa.
TLDR
The findings of this study support the medicinal usefulness of A. rugosa and indicate targets for increasing tilianin and rosmarinic acid production in this plant.
Tyrosine Aminotransferase Contributes to Benzylisoquinoline Alkaloid Biosynthesis in Opium Poppy1[W]
TLDR
The modest correlation between transcript levels and BIA accumulation in opium poppy supports a role for TyrAT in the generation of alkaloid precursors, but it also suggests the occurrence of other sources for 4-hydroxyphenylacetaldehyde.
Jasmonate is involved in the induction of tyrosine aminotransferase and tocopherol biosynthesis in Arabidopsis thaliana
TLDR
Jasmonate plays an important role in the signal transduction pathway regulating TAT activity and the biosynthesis of its product tocopherol, which is known to be radical scavengers in plants.
Proposed biosynthetic pathway for rosmarinic acid in cell cultures of Coleus blumei Benth
TLDR
A biosynthetic pathway for rosmarinic acid is proposed, deduced from studies of the enzymes detectable in preparations from suspension cells of Coleus blumei, which introduces the hydroxyl groups at positions 3 and 3′ of the aromatic rings of the ester 4-coumaroyl-4′-hydroxyphenyllactate giving rise to ros marinic acid.
Enzymes of phenylpropanoid metabolism in the important medicinal plant Melissa officinalis L.
TLDR
Two enzymes of the general phenylpropanoid pathway, phenylalanine ammonia-lyase (PAL) and 4-coumarate:coenzyme A-ligase (4CL), were investigated in suspension cultures of lemon balm and southern-blot analysis suggested the presence of most probably two gene copies in the M. officinalis genome.
Metabolomics Analysis and Biosynthesis of Rosmarinic Acid in Agastache rugosa Kuntze Treated with Methyl Jasmonate
TLDR
Both phenolic acids and precursors for the phenylpropanoid biosynthetic pathway, such as aromatic amino acids and shikimate, were induced as a response to MeJA treatment, and MeJA appears to have an important impact on RA accumulation.
Hydroxyphenylpyruvate Reductase From Cell Suspension Cultures Of Coleus Blumei Benth.
TLDR
Cell suspension cultures of Coleus blumei Benth producing high amounts of rosmarinic acid were used to study the biosynthetic pathway of this caffeic acid ester and the hydroxyphenylpyruvate reductase (HPPR) is characterized in this paper.
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