Molecular Characterization of a New Corneal Pathogen

  title={Molecular Characterization of a New Corneal Pathogen},
  author={Dolena R. Ledee and John DuVall Hay and Thomas J. Byers and David V. Seal and Colin M. Kirknessf},
Purpose. Acanthamoeba was isolated from the cornea of a soft contact lense wearer who had keratitis. The protozoan was also isolated from the contact lens storage case and the domestic water supply used to clean the case. Using morphologic features, all three isolates were identified tentatively as A. griffini, a species not previously associated with keratitis. Complete small subunit ribosomal RNA gene (18S rDNA) sequence analysis was used to characterize further the three isolates. Methods… CONTINUE READING