Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan

  title={Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan},
  author={Yuliya Kleschenko and Danyil Grybchuk and N. Matveeva and Diego H Macedo and Evgeny N Ponirovsky and Alexander N. Lukashev and Vyacheslav Yurchenko},
Here we report sequence and phylogenetic analysis of two new isolates of Leishmania RNA virus 2 (LRV2) found in Leishmania major isolated from human patients with cutaneous leishmaniasis in south Uzbekistan. These new virus-infected flagellates were isolated in the same region of Uzbekistan and the viral sequences differed by only nineteen SNPs, all except one being silent mutations. Therefore, we concluded that they belong to a single LRV2 species. New viruses are closely related to the LRV2… 
Presence and diversity of Leishmania RNA virus in an old zoonotic cutaneous leishmaniasis focus, northeastern Iran: haplotype and phylogenetic based approach.
  • R. Saberi, M. Fakhar, +6 authors A. Bagheri
  • Medicine, Biology
    International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
  • 2020
The results indicated the highest occurrence of Leishmania/LRV2 co-circulation in this known ZCL focus from northeastern Iran and offered an insight into LRV2 haplotype, that can be used for genetic research of LRV 2 in other regions.
Analyses of Leishmania-LRV Co-Phylogenetic Patterns and Evolutionary Variability of Viral Proteins
Leishmania spp. are important pathogens causing a vector-borne disease with a broad range of clinical manifestations from self-healing ulcers to the life-threatening visceral forms. Presence of
Relationship of Leishmania RNA Virus (LRV) and treatment failure in clinical isolates of Leishmania major
Leishmaniasis is caused by different Leishmania spp. Treatment failure (TF) of cutaneous leishmaniasis (CL) is a serious issue that may be due to various reasons, previous studies suggested
Characterization of a new Leishmania major isolate for use in a controlled human infection model
This study addresses a major roadblock in the development of vaccines for leish maniasis, providing a key resource for CHIM studies of sand fly transmitted cutaneous leishmaniasis.
The Maze Pathway of Coevolution: A Critical Review over the Leishmania and Its Endosymbiotic History
There are strong indications that viruses that infect Leishmania spp.
Euglenozoa: taxonomy, diversity and ecology, symbioses and viruses
An updated taxonomy of eugenozoa is presented, complemented by photos of representative species, with notes on diversity, distribution and biology of euglenozoans and a significantly modified taxonomy for kinetoplastids is proposed.


Prevalence and Distribution of Leishmania RNA Virus 1 in Leishmania Parasites from French Guiana.
The aim of this study was to determine the prevalence distribution of LRV1 in Leishmania isolates in French Guiana given that, in this French overseas department, most Leishmaniasis infections are due to these parasite species.
Detection and molecular identification of leishmania RNA virus (LRV) in Iranian Leishmania species
The aim of this study was to survey the presence of LRV in various Iranian Leishmania species originating from patients and animal reservoir hosts and to elucidate the potential role of LRVs in pathogenesis or treatment failure of Old World leishmaniasis.
The complete sequence of Leishmania RNA virus LRV2-1, a virus of an Old World parasite strain.
Sequence analyses show that LRV2-1 differs significantly from members of the LRV1 genus which infect New World parasites, and support a view that transmission of LRV is strictly vertical and suggest thatLRV predate the divergence of Old and New World parasite.
Phylogenetic analysis of Leishmania RNA virus and Leishmania suggests ancient virus-parasite association.
It is concluded that the infection of Leishmania with LRV pre-dates the divergence ofLeishmania into different lineages, and genetic distances between LRV types mirror the heterogeneity between parasite fingerprints and are clustered according to the geographical origin of the strains.
Unraveling the genetic diversity and phylogeny of Leishmania RNA virus 1 strains of infected Leishmania isolates circulating in French Guiana
The first-ever estimate of LRV1 genomic diversity that exists in Leishmania (V.) guyanensis parasites is presented, and cases of multipleLRV1 infections in some parasites are identified, marking a major impact from a clinical viewpoint and for the management of Leishmaniasis-infected patients.
First Report and In Silico Analysis of Leishmania virus (LRV2) identified in an autochthonous Leishmania major isolate in Turkey.
The first autochthonous case of cutaneous leishmaniasis caused by LRV-positive Leishmania major is reported, using conventional PCR targeting the viral capsid protein of LRV.
RNA viruses in trypanosomatid parasites: a historical overview
A historical overview of viruses of trypanosomatids is presented, starting with early reports of virus-like particles on electron microphotographs, and culminating in detailed molecular descriptions of viruses obtained using modern next generation sequencing-based techniques.
Leptomonas seymouri narna-like virus 1 and not leishmaniaviruses detected in kala-azar samples from India
It is shown that the human host is exposed to an RNA virus in LS, another coinfecting parasite with LD, i.e., the “LD-LS-Lepsey NLV1 triple pathogen” phenomenon, unveils a new paradigm of research towards revisiting the mysteries of Indian leishmaniasis pathogenesis and management.
RNA Viruses in Blechomonas (Trypanosomatidae) and Evolution of Leishmaniavirus
Evolution of leishmaniaviruses is more complex than previously thought and includes occasional host switching, and evidence that even representatives of the family Narnaviridae are capable of host switching and evidently have accomplished switches multiple times in the course of their evolution.
Association of the Endobiont Double-Stranded RNA Virus LRV1 With Treatment Failure for Human Leishmaniasis Caused by Leishmania braziliensis in Peru and Bolivia.
The association of LRV1 with clinical drug treatment failure could serve to guide more-effective treatment of tegumentary disease caused by L. braziliensis, and there was no significant association with intrinsic Sb(V) resistance among parasites, suggesting that treatment failure arises fromLRV1-mediated effects on host metabolism and/or parasite survival.