• Chemistry
  • Published 1976

Molecular Basis ofO-Galactosidase a-Complementation

@inproceedings{Villarejo1976MolecularBO,
  title={Molecular Basis ofO-Galactosidase a-Complementation},
  author={Merna R. Villarejo and Audree V. Fowler and Patrice J. Zamenhof},
  year={1976}
}
Inprevious studies, a cyanogenbromide peptidederived from amino-acid residues 3-92of f3- galactosidase (EC3.2.1.23; ,1-D-galactoside galactohydro- lase) wasshowntohavea-donor activity inintracistronic a-complementation. We havenow isolated thedefective fl-galactosidase a-acceptor proteinfrom the deletion mutantstrain M15ofEscherichia coliandfindthatit lacksresidues 11-41off3-galactosidase. Thisisdemon- strated bytheisolation andsequence determination ofa cyanogen bromidepeptide… CONTINUE READING