Molecular Architecture of the Yeast Monopolin Complex.

  title={Molecular Architecture of the Yeast Monopolin Complex.},
  author={Kevin D Corbett and Stephen C Harrison},
  journal={Cell reports},
  volume={1 6},
The Saccharomyces cerevisiae monopolin complex directs proper chromosome segregation in meiosis I by mediating co-orientation of sister kinetochores on the meiosis I spindle. The monopolin subunits Csm1 and Lrs4 form a V-shaped complex that may directly crosslink sister kinetochores. We report here biochemical characterization of the monopolin complex subunits Mam1 and Hrr25 and of the complete four-protein monopolin complex. By purifying monopolin subcomplexes with different subunit… CONTINUE READING