Molecular Analysis of the rDNA ITS-1 Intergenic Spacer in Drosophila mulleri, D. arizonae, and Their Hybrids

@article{Baffi2002MolecularAO,
  title={Molecular Analysis of the rDNA ITS-1 Intergenic Spacer in Drosophila mulleri, D. arizonae, and Their Hybrids},
  author={Milla Alves Baffi and Carlos Roberto Ceron},
  journal={Biochemical Genetics},
  year={2002},
  volume={40},
  pages={411-421}
}
We analyzed the ITS-1 spacer region of the rDNA in Drosophila mulleri and D. arizonae, two sibling species belonging to the mulleri complex (repleta group) and in hybrids obtained in both cross directions. In spite of several previous studies showing the incompatibility of crosses involving D. arizonae females and D. mulleri males, we were able to obtain hybrids in this direction. Complete ITS-1 region was amplified using primers with homology at the 3′-end of the 18S rDNA and the 5′-end of the… CONTINUE READING