Moisture-induced aggregation of lyophilized proteins in the solid state.

@article{Liu1991MoistureinducedAO,
  title={Moisture-induced aggregation of lyophilized proteins in the solid state.},
  author={Wei Liu and Robert Langer and Alexander M. Klibanov},
  journal={Biotechnology and bioengineering},
  year={1991},
  volume={37 2},
  pages={
          177-84
        }
}
A critical problem in the storage and delivery of pharmaceutical proteins is their aggregation induced by moisture. A model system has been elaborated and investigated to elucidate the mechanism of this phenomenon. When 10 mg of bovine serum albumin lyophilized from an aqueous solution of pH 7.3 are wetted with just 3 muL of a buffered physiological saline solution and incubated in the solid state at 37 degrees C, the protein progressively loses its solubility in water; e.g., after a 24 h… CONTINUE READING
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