Modulation of the hepatitis C virus RNA-dependent RNA polymerase activity by the non-structural (NS) 3 helicase and the NS4B membrane protein.

@article{Piccininni2002ModulationOT,
  title={Modulation of the hepatitis C virus RNA-dependent RNA polymerase activity by the non-structural (NS) 3 helicase and the NS4B membrane protein.},
  author={Sabina Piccininni and Agoritsa Varaklioti and M. Nardelli and Bhuvanesh Dave and Kevin D. Raney and John G. McCarthy},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 47},
  pages={45670-9}
}
The hepatitis C virus (HCV) nonstructural protein 5B (NS5B) is believed to be the central catalytic enzyme responsible for HCV replication but there are many unanswered questions about how its activity is controlled. In this study we reveal that two other HCV proteins, NS3 (a protease/helicase) and NS4B (a hydrophobic protein of unknown function), physically and functionally interact with the NS5B polymerase. We describe a new procedure for generating highly pure NS4B, and use this protein in… CONTINUE READING