Modulation of the connexin26 tumor suppressor gene expression through methylation in human mammary epithelial cell lines.

@article{Singal2000ModulationOT,
  title={Modulation of the connexin26 tumor suppressor gene expression through methylation in human mammary epithelial cell lines.},
  author={Rakesh Singal and Zheng Jin Tu and Jane M Vanwert and Gordon D. Ginder and David T. Kiang},
  journal={Anticancer research},
  year={2000},
  volume={20 1A},
  pages={59-64}
}
BACKGROUND Normal mammary epithelial cells express mainly gap junction connexin 26 (Cx26) that is either reduced or absent in breast cancers. Since connexin gene mutations are rare we examined if Cx26 gene repression is related to hypermethylation. MATERIALS AND METHODS Five breast epithelial cell lines were examined for Cx26 mRNA expression and hypermethylation. Treatment with a DNA methyltransferase inhibitor, 5-Aza-2'-deoxycytidine (5-Aza-CdR), was carried out to determine if Cx26 gene… CONTINUE READING