Modulation of steroidogenic gene expression and hormone production of H295R cells by pharmaceuticals and other environmentally active compounds.

@article{Gracia2007ModulationOS,
  title={Modulation of steroidogenic gene expression and hormone production of H295R cells by pharmaceuticals and other environmentally active compounds.},
  author={Tannia Gracia and Kl{\'a}ra Hilscherov{\'a} and Paul D Jones and John L. Newsted and Eric B Higley and Xiaowei Zhang and Markus Hecker and Margaret Burkhardt Murphy and Richard M K Yu and Paul K S Lam and Rudolf S S Wu and John Paul Giesy},
  journal={Toxicology and applied pharmacology},
  year={2007},
  volume={225 2},
  pages={142-53}
}
The H295R cell bioassay was used to evaluate the potential endocrine disrupting effects of 18 of the most commonly used pharmaceuticals in the United States. Exposures for 48 h with single pharmaceuticals and binary mixtures were conducted; the expression of five steroidogenic genes, 3betaHSD2, CYP11beta1, CYP11beta2, CYP17 and CYP19, was quantified by Q-RT-PCR. Production of the steroid hormones estradiol (E2), testosterone (T) and progesterone (P) was also evaluated. Antibiotics were shown to… CONTINUE READING