Modulation of pteroylpolyglutamate concentration and length in response to altered folate nutrition in a comprehensive range of rat tissues.

@article{Ward1990ModulationOP,
  title={Modulation of pteroylpolyglutamate concentration and length in response to altered folate nutrition in a comprehensive range of rat tissues.},
  author={G J Ward and Peter F. Nixon},
  journal={The Journal of nutrition},
  year={1990},
  volume={120 5},
  pages={476-84}
}
For a range of rat tissue extracts, the concentrations of total folates and of short-chain pteroylpolyglutamates were assayed by Lactobacillus casei with and without conjugase treatment, respectively, and the concentration and chain length of H4PteGlnn and 5,10-CH2-H4PteGlnn together were assayed after binding to thymidylate synthase and tritiated fluorodeoxyuridylate. For rats fed a nonpurified diet and consuming 26 micrograms of folic acid daily, the respective concentrations of these total… CONTINUE READING